SETD8 antibody (N-Term)

Catalog No. ABIN356375

The Mouse Monoclonal anti-SETD8 antibody is suitable to detect SETD8 in samples from Human and Mouse. It has been validated for WB and EIA.

Quick Overview for SETD8 antibody (N-Term) (ABIN356375)

Target: SETD8 (SET Domain Containing (Lysine Methyltransferase) 8 (SETD8))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This SETD8 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Clone: 43AT890

Product Details anti-SETD8 Antibody

Binding Specificity: N-Term

Specificity: This antibody is specific to SET07.

Characteristics: Synonyms: SET8, PRSET7, PR-Set7, KMT5A, Histone-lysine N-methyltransferase SETD8, H4-K20-HMTaseSETD8, SET domain-containing protein 8, PR/SET domain-containing protein 07, LysineN-methyltransferase 5A

Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody was raised using purified recombinant GST fusion protein encodingN-terminal of human SET07.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for SETD8

Target: SETD8 (SET Domain Containing (Lysine Methyltransferase) 8 (SETD8))

Alternative Name: SETD8 / SET07

Background: SET07 is a histone methyltransferase that methylates Lys-20 of histone H4. H4 Lys-20 methylation represents a specific tag for epigenetic transcriptional repression. The nuclear SET07 protein, which associates with silent chromatin on euchromatic arms but shows no association with constitutive heterochromatin, prefers nucleosomes as substrate compared to free histones. It appears that SET07 may play a role in maintaining silent chromatin by preventing neighboring acetylation of H4 tail. Although the SET domain contains the active site of enzymatic activity, both sequences upstream and downstream of the SET domain are required for methyltransferase activity.Synonyms: H4-K20-HMTase SETD8, Histone-lysine N-methyltransferase SETD8, KMT5A, Lysine N-methyltransferase 5A, PR-Set7, PR/SET domain-containing protein 07, PRSET7, SET domain-containing protein 8, SET8

Gene ID: 387893

UniProt: Q9NQR1