Nanog antibody
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- Target See all Nanog (NANOG) Antibodies
- Nanog (NANOG) (Nanog Homeobox (NANOG))
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Reactivity
- Human
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Host
- Mouse
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Clonality
- Monoclonal
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Conjugate
- This Nanog antibody is un-conjugated
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Application
- Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)
- Specificity
- This antibody is specific to Human NANOG.
- Cross-Reactivity (Details)
- Species reactivity (tested):Human.
- Characteristics
- Synonyms: Homeobox protein NANOG
- Purification
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS
- Immunogen
- This antibody is generated from mouse immunized with human recombinant NANOGprotein.
- Clone
- 60CT70-5-12
- Isotype
- IgG1
- Top Product
- Discover our top product NANOG Primary Antibody
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- Application Notes
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ELISA: 1/1,000. Western blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. - Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- 0.25 mg/mL
- Buffer
- PBS, 0.09 % (W/V) Sodium Azide
- Preservative
- Sodium azide
- Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
- Storage
- 4 °C/-20 °C
- Storage Comment
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Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch. - Expiry Date
- 12 months
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- Target
- Nanog (NANOG) (Nanog Homeobox (NANOG))
- Alternative Name
- NANOG (NANOG Products)
- Synonyms
- 2410002E02Rik antibody, ENK antibody, ecat4 antibody, NANOG antibody, hacp antibody, wu:fd19e04 antibody, wu:fd20a07 antibody, zgc:193933 antibody, Nanog homeobox antibody, nanog homeobox antibody, Nanog antibody, NANOG antibody, nanog antibody
- Background
- NANOG is a transcription regulator involved in inner cell mass and embryonic stem (ES) cell proliferation and self-renewal. It imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages. This protein blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. NANOG acts as a transcriptional activator or repressor. It binds optimally to the DNA consensus sequence 5'-[CG][GA][CG]C[GC]ATTAN[GC]-3'. When overexpressed, this protein promotes cells to enter into S phase and proliferation.Synonyms: Homeobox protein NANOG
- Molecular Weight
- 34620 Da
- Gene ID
- 79923
- UniProt
- Q9H9S0
- Pathways
- Stem Cell Maintenance
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