NCOA1 antibody

Catalog No. ABIN356411

The Mouse Monoclonal anti-NCOA1 antibody has been validated for WB, IP, IHC (p) and EIA. It is suitable to detect NCOA1 in samples from Human and Mouse.

Quick Overview for NCOA1 antibody (ABIN356411)

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This NCOA1 antibody is un-conjugated

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Clone: 17AT28

Product Details anti-NCOA1 Antibody

Specificity: This antibody is specific to Src.

Characteristics: Synonyms: c-Src, pp60c-src, Proto-oncogene tyrosine-protein kinase Src, Proto-oncogene c-Src

Purification: Protein G Chromatography eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from mouse immunized with a recombinant protein of fulllength human SRC.

Isotype: IgG1

Application Details

Application Notes: ELISA: 1/1,000Western blotting: 1/100-1/500Immunohistochemistry: 1/50-1/100Immunoprecipitation: 2 μg/test.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing.
Shelf life: one year from despatch.

Expiry Date: 12 months

Target Details for NCOA1

Target: NCOA1 (Nuclear Receptor Coactivator 1 (NCOA1))

Alternative Name: SRC1

Background: The gene for SRC is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. SRC, the protein encoded by this gene, is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in SRC could be involved in the malignant progression of colon cancer.Synonyms: Proto-oncogene c-Src, Proto-oncogene tyrosine-protein kinase Src, c-Src, pp60c-src

Gene ID: 6714

UniProt: P12931

Pathways: Intracellular Steroid Hormone Receptor Signaling Pathway, Nuclear Hormone Receptor Binding, Chromatin Binding, Regulation of Lipid Metabolism by PPARalpha