EPHX2 antibody (Internal Region)

Catalog No. ABIN356443

This Rabbit Polyclonal antibody specifically detects EPHX2 in IHC (p). It exhibits reactivity toward Human.

Quick Overview for EPHX2 antibody (Internal Region) (ABIN356443)

Target: EPHX2 (Epoxide Hydrolase 2, Cytoplasmic (EPHX2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPHX2 antibody is un-conjugated

Application: Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-EPHX2 Antibody

Binding Specificity: Internal Region

Specificity: This antibody reacts with Soluble Epoxide Hydrolase (EPHX2).

Cross-Reactivity (Details): Species reactivity (tested):Human.

Purification: Immunoaffinity Chromatography.

Immunogen: Synthetic peptide - KLH conjugated corresponding to internal domain of Human EPHX2.

Isotype: IgG

Application Details

Application Notes: Immunohistochemistry on Paraffin Embedded Tissues: 20 μg/mL. This EPHX2 antibody was validated for use in immunohistochemistry on a panel of 21formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigenretrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides wereincubated with biotinylated secondary antibody LS-D1, followed by alkalinephosphatase-streptavidin and chromogen.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Concentration: 1.0 mg/mL

Buffer: PBS containing 0.09 % Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at -20 °C to -70 °C for longer.

Target Details for EPHX2

Target: EPHX2 (Epoxide Hydrolase 2, Cytoplasmic (EPHX2))

Alternative Name: Epoxide Hydrolase 2 / EPHX2

Background: Soluble Epoxide Hydrolase (sEH) also known as cytosolic Epoxide Hydrolase is a phase I xenobiotic-metabolizing enzyme that converts epoxyeicosatrienoic acids (EETs) into their corresponding dihydroxyeicosatrienoic acids (DHETs). EETs are thought to be involved in vascular tone, ion channel regulation, mitogenesis and cell signaling. DHETs have, in general, less biological activity then EETs and are more readily excreted, thus hydration of EETs by sEH maybe a means of attenuating or eliminating their activity. sEHs have been identified in many tissues including liver, kidney, adrenal, pancreatic islets, gi tract and vascular smooth muscle.Synonyms: CEH, Cytosolic epoxide hydrolase, Epoxide hydratase, SEH, Soluble epoxide hydrolase

Gene ID: 2053

NCBI Accession: NP_001970

UniProt: P34913