JMJD4 antibody (C-Term)

Catalog No. ABIN356562

Product Details anti-JMJD4 Antibody

Target: JMJD4 (Jumonji Domain Containing 4 (JMJD4))

Binding Specificity: C-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This JMJD4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody will recognize JMJD4 (C-term). It Cross reacts with Mouse (Predicted by 100 % Antigen Homology).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human JMJD4.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/250-1/500. Immunohistochemistry: 1/10approx. 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS containing 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for JMJD4

Target: JMJD4 (Jumonji Domain Containing 4 (JMJD4))

Alternative Name: JMJD4 (JMJD4 Products)

Synonyms: JMJD4 antibody, 6430559I23 antibody, RGD1307186 antibody, jumonji domain containing 4 antibody, jumonji domain containing 4 L homeolog antibody, JMJD4 antibody, jmjd4.L antibody, Jmjd4 antibody

Background: Covalent modification of histones plays critical role in regulating chromatin structure and transcription. While most covalent histone modifications are reversible, only recently has it been established that methyl groups are subject to enzymatic removal from histones. A family of novel JmjC domain-containing histone demethylation (JHDM) enzymes have been identified that perform this specific function. Histone demethylation by JHDM proteins requires cofactors Fe(II) and alpha-ketoglutarate. Family members include JHDM1 (demethylating histone 3 at lysine 36), and JHDM2A as well as JMJD2CH3K9 (both of which demethylate histone 3 at lysine 9). Contributions of histone demethylase activity to tumor development, decreases in cell proliferation, and hormone-dependent transcriptional activation have been observed.Synonyms: JmjC domain-containing protein 4, Jumonji domain-containing protein 4

Gene ID: 65094, 9606

UniProt: Q9H9V9