CD4 antibody (N-Term)

Catalog No. ABIN357033

This anti-CD4 antibody is a Rabbit Polyclonal antibody detecting CD4 in WB and IHC (p). Suitable for Human.

Quick Overview for CD4 antibody (N-Term) (ABIN357033)

Target: CD4

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CD4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-CD4 Antibody

Binding Specificity: N-Term

Specificity: This antibody detects CD4 at N-term.

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. Then the antibody is purified by peptide affinity purification.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human CD4.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 50 - 1: 100. Immunohistochemistry 1: 10 - 1: 50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for CD4

Target: CD4

Alternative Name: CD4

Background: CD4 is a single chain transmembraneous glycoprotein (59 kDa) which belongs to the immunoglobulin superfamily. CD4 is present on a subset of T lymphocytes (helper/inducer T cells) and is also expressed at a lower level on monocytes, tissue macrophages and granulocytes. The antigen is involved in binding to MHC class II molecules. The intracellular domain of the antigen is associated with p56lck protein tyrosine kinase.Synonyms: T-cell surface antigen T4/Leu-3, T-cell surface glycoprotein CD4

Molecular Weight: 51111 Da

Gene ID: 920, 9606

UniProt: P01730

Pathways: TCR Signaling, Maintenance of Protein Location, CXCR4-mediated Signaling Events