NMD3 antibody (C-Term)

Catalog No. ABIN357284

The Rabbit Polyclonal anti-NMD3 antibody is suitable to detect NMD3 in samples from Human and Mouse. It has been validated for WB and EIA.

Quick Overview for NMD3 antibody (C-Term) (ABIN357284)

Target: NMD3 (60S Ribosomal Export Protein NMD3 (NMD3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This NMD3 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-NMD3 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects NMD3 at C-term.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human NMD3.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 100 - 1: 500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for NMD3

Target: NMD3 (60S Ribosomal Export Protein NMD3 (NMD3))

Alternative Name: NMD3

Background: It has been suggested that NMD3 is a cytoplasmic factor required for a late cytoplasmic assembly step of the 60S subunit but is not a ribosomal protein. A mutation in NMD3 was found to be lethal in the absence of XRN1, which encodes the major cytoplasmic exoribonuclease responsible for mRNA turnover. The NMD3 protein sequence does not contain readily recognizable motifs of known function. However, NMD3 orthologues display an amino-terminal domain containing four repeats of Cx2C, reminiscent of zinc-binding proteins, implicated in nucleic acid binding or protein oligomerization.Synonyms: 60S ribosomal export protein NMD3, CGI-07, hNMD3

Molecular Weight: 57603 Da

Gene ID: 51068, 9606

UniProt: Q96D46