SLUG antibody (N-Term)

Catalog No. ABIN357448

Product Details anti-SLUG Antibody

Target: SLUG (SNAI2) (Snail Family Zinc Finger 2 (SNAI2))

Binding Specificity: N-Term

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SLUG antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Specificity: This antibody detects SLUG at N-term.

Purification: Prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human SLUG.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

References for anti-SLUG antibody (ABIN357448)

Liang, Wang, Li, Yu, Zhang, Li: "miR-33a suppresses the nuclear translocation of ?-catenin to enhance gemcitabine sensitivity in human pancreatic cancer cells." in: Tumour biology, (2015) (PubMed).

Huang, Chen, Chang, Yuan, Jeng: "Hepatocyte growth factor activates Wnt pathway by transcriptional activation of LEF1 to facilitate tumor invasion." in: Carcinogenesis, Vol. 33, Issue 6, pp. 1142-8, (2012) (PubMed).

Collado, Thiede, Baker, Askew, Igbani, Corwin: "The postnatal accumulation of junctional E-cadherin is inversely correlated with the capacity for supporting cells to convert directly into sensory hair cells in mammalian balance organs." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 31, Issue 33, pp. 11855-66, (2011) (PubMed).

Fu, Lv, Lin, Wu, Wang, Zhou, Zhang, Wang, Tsang, Zhu, Wang: "Ubiquitin ligase cullin 7 induces epithelial-mesenchymal transition in human choriocarcinoma cells." in: The Journal of biological chemistry, Vol. 285, Issue 14, pp. 10870-9, (2010) (PubMed).

Bitoun, Oliver, Davies: "The mixed-lineage leukemia fusion partner AF4 stimulates RNA polymerase II transcriptional elongation and mediates coordinated chromatin remodeling." in: Human molecular genetics, Vol. 16, Issue 1, pp. 92-106, (2007) (PubMed).

Gautier, Tietge, Boverhof, Perton, Le Guern, Masson, Rensen, Havekes, Lagrost, Kuipers: "Hepatic lipid accumulation in apolipoprotein C-I-deficient mice is potentiated by cholesteryl ester transfer protein." in: Journal of lipid research, Vol. 48, Issue 1, pp. 30-40, (2006) (PubMed).

Target Details for SLUG

Target: SLUG (SNAI2) (Snail Family Zinc Finger 2 (SNAI2))

Alternative Name: SNAI2 / SLUG (SNAI2 Products)

Synonyms: Slug antibody, Slugh antibody, Snail2 antibody, snail family transcriptional repressor 2 antibody, snail family zinc finger 2 antibody, Snai2 antibody

Background: SLUG is a member of the Snail family of C2H2-type zinc finger transcription factors. This protein acts as a transcriptional repressor that binds to E-box motifs and is also likely to repress E-cadherin transcription in breast carcinoma. This protein is involved in epithelial-mesenchymal transitions and has antiapoptotic activity. Mutations in the gene encoding SLUG may be associated with sporadic cases of neural tube defects.Synonyms: Neural crest transcription factor Slug, Protein snail homolog 2, SLUGH, Zinc finger protein SNAI2

Molecular Weight: 29986 Da

Gene ID: 6591, 9606

UniProt: O43623