GPLD1 antibody (C-Term)

Catalog No. ABIN357724

This Rabbit Polyclonal antibody specifically detects GPLD1 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for GPLD1 antibody (C-Term) (ABIN357724)

Target: GPLD1 (Glycosylphosphatidylinositol Specific phospholipase D1 (GPLD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This GPLD1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-GPLD1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects GPLD1 (C-term).

Purification: Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human GPLD1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for GPLD1

Target: GPLD1 (Glycosylphosphatidylinositol Specific phospholipase D1 (GPLD1))

Alternative Name: GPLD1 / GPI-PLD

Background: GPLD1 is expressed in numerous tissues and cells and specifically cleaves GPI-anchored proteins. Liver has the highest level of GPI-PLD expression and is the primary organ contributing to GPLD1 in the serum. GPLD1 is abundant in serum in which it associates with polipoproteins AI and AIV. Increased serum GPLD1 is associated with insulin resistance and elevated serum triglycerides. Many surface proteins are attached to eukaryotic cell membranes via glycosylphosphatidylinositol (GPI) anchors that are covalently bound to the C-terminus of the protein and cleavage of the GPI moiety by GPLD1, only enzyme known that cleavage GPI anchor, may represent a means of regulating attachment of these proteins to the cell surface, or alternatively, their release into the extracellular environment.Synonyms: GPI-specific phospholipase D, Glycoprotein phospholipase D, Glycosyl-phosphatidylinositol-specific phospholipase D, PI-G PLD, PIGPLD1, Phosphatidylinositol-glycan-specific phospholipase D

Molecular Weight: 92666 Da

Gene ID: 2822, 9606

UniProt: P80108

Pathways: Inositol Metabolic Process, Regulation of Carbohydrate Metabolic Process