HSF1 antibody (Sumoylation Site)
Quick Overview for HSF1 antibody (Sumoylation Site) (ABIN357730)
Target
See all HSF1 AntibodiesReactivity
Host
Clonality
Conjugate
Application
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Binding Specificity
- Sumoylation Site
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Specificity
- This antibody detects HSF1 Sumoylation Site.
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Purification
- Protein G Chromatography, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.
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Immunogen
- This antibody is generated from rabbits immunized with the KLH conjugated synthetic peptide PLVRVKEEPPSPPQSPR containing a predicted sumoylation site from the central region of human HSF1.
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Isotype
- Ig Fraction
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anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 594)
HSF1 Reactivity: Mouse WB, IHC, ELISA, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 Atto 594
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 390)HSF1 Reactivity: Mouse WB, IHC, ELISA, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 Atto 390
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Atto 488)HSF1 Reactivity: Mouse WB, IHC, ELISA, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 Atto 488
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (Biotin)HSF1 Reactivity: Mouse WB, IHC, ELISA, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 Biotin
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (FITC)HSF1 Reactivity: Mouse WB, IHC, ELISA, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 FITC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (APC)HSF1 Reactivity: Mouse WB, IHC, ELISA, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 APC
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (HRP)HSF1 Reactivity: Mouse WB, IHC, ELISA, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 HRP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PerCP)HSF1 Reactivity: Mouse WB, IHC, ELISA, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 PerCP
anti-Heat Shock Factor Protein 1 (HSF1) (AA 378-395) antibody (PE)HSF1 Reactivity: Mouse WB, IHC, ELISA, IF, IP, ICC, EMSA Host: Rat Monoclonal 10H8 PE
anti-Heat Shock Factor Protein 1 (HSF1) antibodyKD Validated HSF1 Reactivity: Human WB, FACS, IF (p) Host: Rabbit Monoclonal 23GB2715 unconjugated Recombinant Antibody
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Application Notes
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ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user. -
Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- 0.25 mg/mL
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Buffer
- PBS with 0.09 % (W/V) Sodium Azide as preservative.
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Preservative
- Sodium azide
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Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
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Handling Advice
- Avoid repeated freezing and thawing.
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Storage
- 4 °C/-20 °C
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Storage Comment
- Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
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- HSF1 (Heat Shock Factor Protein 1 (HSF1))
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Alternative Name
- Heat Shock Factor 1 / HSF1
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Background
- Heat shock transcription factor 1 (HSF1) mediates the induction of heat shock protein gene expression in cells exposed to elevated temperature and other stress conditions. In response to stress, HSF1 acquires DNA-binding ability and localizes to nuclear stress granules. SUMO modification of HSF1 converts HSF1 to the DNA-binding form. HSF1 colocalizes with SUMO-1 in nuclear stress granules, which is prevented by mutation of the HSF1 lysine targeted for sumoylation.Synonyms: HSF-1, HSTF1, Heat shock transcription factor 1
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Molecular Weight
- 57260 Da
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Gene ID
- 3297, 9606
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UniProt
- Q00613
Target
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