PAPSS1 antibody (C-Term)

Catalog No. ABIN357814

This Rabbit Polyclonal antibody specifically detects PAPSS1 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for PAPSS1 antibody (C-Term) (ABIN357814)

Target: PAPSS1 (3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAPSS1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PAPSS1 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects PAPSS1 (C-term).

Purification: Purified

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PAPSS1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PAPSS1

Target: PAPSS1 (3'-phosphoadenosine 5'-phosphosulfate Synthase 1 (PAPSS1))

Alternative Name: PAPSS1

Background: Sulfotransferase (SULT) enzymes catalyze the sulfate conjugation of many drugs, xenobiotic compounds, hormones, and neurotransmitters. 3'-phosphoadenosine 5'-phosphosulfate (PAPS) synthase (PAPSS) catalyzes the biosynthesis of PAPS which serves as the universal sulfonate donor compound for all sulfotransferase reactions. In humans, PAPS is synthesized from adenosine 5-prime triphosphate (ATP) and inorganic sulfate by 2 isoforms, PAPSS1 and PAPSS2 (603005). Bifunctional PAPSS1 is comprised of an N-terminal APS kinase domain, and a C-terminal ATP sulfurylase domain. Full-length protein has significantly less APS kinase activity than the N-terminal fragment, suggesting that the C-terminal domain exerts a regulatory role on the N-terminal APS kinase activity. In humans there are two major isoforms: PAPSS1 and PAPSS2. In brain and skin PAPSS1 is the major isoform, whereas in liver, cartilage and adrenal glands PAPSS2 isoform expression dominates. The predicted 623-amino acid protein is 98 % identical to mouse PAPS synthase. The N-terminal 268-amino acid region of human PAPS synthase resembles APS kinases from other organisms and contains 3 conserved nucleotide-binding motifs.Synonyms: ATPSK1, Bifunctional 3'-phosphoadenosine 5'-phosphosulfate synthase 1, PAPSS, PAPSS 1, SK1, Sulfurylase kinase 1

Molecular Weight: 70833 Da

Gene ID: 9061, 9606

UniProt: O43252

Pathways: Glycosaminoglycan Metabolic Process, Ribonucleoside Biosynthetic Process