BID antibody (pSer65)

Catalog No. ABIN358089

The Rabbit Polyclonal anti-BID antibody (ABIN358089) specifically detects BID in WB, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for BID antibody (pSer65) (ABIN358089)

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This BID antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-BID Antibody

Binding Specificity: pSer65

Specificity: This antibody detects BID pSer65.

Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S65 of human Bid.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for BID

Target: BID (BH3 Interacting Domain Death Agonist (BID))

Alternative Name: BID

Background: Bid is a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. Bid induces ICE-like proteases and apoptosis. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8), CASP8 cleaves this encoded protein, and the major proteolytic product p15 Bid translocates to mitochondria where it triggers cytochrome c release.Synonyms: BH3-interacting domain death agonist, p22 BID

Molecular Weight: 26836 Da

Gene ID: 637

NCBI Accession: NP_001187

UniProt: P55957

Pathways: Apoptosis, Caspase Cascade in Apoptosis, Positive Regulation of Endopeptidase Activity