C-JUN antibody (pSer63)

Catalog No. ABIN358110

This anti-C-JUN antibody is a Rabbit Polyclonal antibody detecting C-JUN in WB, IHC (p) and EIA. Suitable for Human.

Quick Overview for C-JUN antibody (pSer63) (ABIN358110)

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This C-JUN antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-C-JUN Antibody

Binding Specificity: pSer63

Specificity: This antibody detects AP-1/c-Jun pSer63.

Purification: Protein G Affinity Chromatography. Then, the antibody fraction is peptide affinity purified in a 2-step procedure with control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S63 of human cJun.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western Blot: 1/100-1/500. Dot Blot: 1/500. Immunohistochemistry: 1/50-1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for C-JUN

Target: C-JUN (JUN) (Jun Proto-Oncogene (JUN))

Alternative Name: AP-1 / C-Jun

Background: The gene for the cJun protein is the putative transforming gene of avian sarcoma virus 17. The protein is highly similar to the viral protein, and interacts directly with specific target DNA sequences to regulate gene expression. The gene for this protein is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.Synonyms: Activator protein 1, Proto-oncogene c-jun, Transcription factor AP1, V-jun avian sarcoma virus 17 oncogene homolog, p39

Molecular Weight: 35676 Da

Gene ID: 3725, 9606

UniProt: P05412

Pathways: MAPK Signaling, RTK Signaling, WNT Signaling, Fc-epsilon Receptor Signaling Pathway, Activation of Innate immune Response, Myometrial Relaxation and Contraction, Skeletal Muscle Fiber Development, Protein targeting to Nucleus, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling, S100 Proteins