RPS6KA3 antibody (pSer369)

Catalog No. ABIN358394

The Rabbit Polyclonal anti-RPS6KA3 antibody has been validated for EIA. It is suitable to detect RPS6KA3 in samples from Human.

Quick Overview for RPS6KA3 antibody (pSer369) (ABIN358394)

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KA3 antibody is un-conjugated

Application: Enzyme Immunoassay (EIA)

Product Details anti-RPS6KA3 Antibody

Binding Specificity: pSer369

Specificity: This antibody detects RPS6KA3 pSer369. Predicted to cross react with Mouse and Zebrafish (100 % Antigen Homology).

Purification: Affinity purification in a 2-step procedure with the control and phosphorylated peptides. The phospho-specific antibody is eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic phosphopeptide corresponding to amino acid residues surrounding S369 of human RPS6KA3.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Dot Blot: 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) Sodium Azide as preservative.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for RPS6KA3

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Alternative Name: RPS6KA3 / RSK2

Background: RSK3 is a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including members of the mitogen-activated kinase (MAPK) signalling pathway. The activity of this protein has been implicated in controlling cell growth and differentiation. Mutations in the gene have been associated with Coffin-Lowry syndrome (CLS).Synonyms: ISPK-1, ISPK1, MAPKAPK-1b, MAPKAPK1, RSK-2, Ribosomal S6 kinase 2, Ribosomal protein S6 kinase alpha-3, S6K-alpha-3, p90RSK3

Molecular Weight: 83736 Da

Gene ID: 6197, 9606

UniProt: P51812

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades