MMP25 antibody (C-Term)

Catalog No. ABIN358695

The Rabbit Polyclonal anti-MMP25 antibody (ABIN358695) specifically detects MMP25 in WB and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for MMP25 antibody (C-Term) (ABIN358695)

Target: MMP25 (Matrix Metallopeptidase 25 (MMP25))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MMP25 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-MMP25 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to MMP25.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MMP25.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for MMP25

Target: MMP25 (Matrix Metallopeptidase 25 (MMP25))

Alternative Name: MMP-25

Background: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMPs are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. However, MMP25 is a member of the membrane-type MMP (MT-MMP) subfamily, each member of this subfamily contains a potential transmembrane domain suggesting that these proteins are expressed at the cell surface rather than secreted. In addition, it is thought that this protein is activated intracellularly by furin-like enzymes. This protein activates MMP2 by cleavage. The gene has previously been referred to as MMP20 but has been renamed matrix metalloproteinase 25 (MMP25).Synonyms: Leukolysin, MMP20, MMP25, MMPL1, MT-MMP 6, MT6-MMP, MT6MMP, Matrix metalloproteinase-25, Membrane-type matrix metalloproteinase 6

Gene ID: 64386, 5874

UniProt: Q9NPA2