PRPS1 antibody (N-Term)

Catalog No. ABIN359137

This Rabbit Polyclonal antibody specifically detects PRPS1 in WB and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for PRPS1 antibody (N-Term) (ABIN359137)

Target: PRPS1 (phosphoribosyl Pyrophosphate Synthetase 1 (PRPS1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PRPS1 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PRPS1 Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to PRPS1/PRPS2/PRPS3.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human PRPS1/2/3.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PRPS1

Target: PRPS1 (phosphoribosyl Pyrophosphate Synthetase 1 (PRPS1))

Alternative Name: PRPS1

Background: Phosphoribosylpyrophosphate synthetase (PRPS, EC 2.7.6.1) catalyzes the phosphoribosylation of ribose 5-phosphate to 5-phosphoribosyl-1-pyrophosphate, which is necessary for the de novo and salvage pathways of purine, pyrimidine, and pyridine biosynthesis. By PCR of human lymphoblast mRNA using primers based on the cDNA sequence of rat PRS I (Prps1), Roessler et al. (1990) isolated a partial human PRPS1 cDNA. They used this partial cDNA to screen lymphoblast cDNA libraries and isolated additional cDNAs corresponding to the entire PRPS1 coding region. The deduced PRPS1 protein has 318 amino acids.Synonyms: PPRibP, PRS-I, Phosphoribosyl pyrophosphate synthetase I, Ribose-phosphate pyrophosphokinase I

Gene ID: 5631, 9606

UniProt: P60891

Pathways: Ribonucleoside Biosynthetic Process