Deoxyguanosine Kinase antibody (N-Term)

Catalog No. ABIN359185

This Rabbit Polyclonal antibody specifically detects Deoxyguanosine Kinase in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for Deoxyguanosine Kinase antibody (N-Term) (ABIN359185)

Target: Deoxyguanosine Kinase (DGUOK)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Deoxyguanosine Kinase antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-Deoxyguanosine Kinase Antibody

Binding Specificity: N-Term

Specificity: This antibody reacts to Deoxyguanosine Kinase (DGUOK).

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the N-terminal region of human DGUOK.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1/1,000. Western blotting: 1/100 - 1/500.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for Deoxyguanosine Kinase

Target: Deoxyguanosine Kinase (DGUOK)

Alternative Name: Deoxyguanosine Kinase

Background: Mitochondrial deoxyguanosine kinase (DGUOK) is required for the phosphorylation of several deoxyribonucleosides and certain purine deoxykribonucleoside analogs widely employed as antiviral and chemotherapeutic agents. Purine deoxyribonucleoside analogs are extensively used in treatment of lymphoproliferative disorders. These compounds are administered as pro-drugs, and their efficiency is dependent on intracellular phosphorylation to the corresponding triphosphates. In mammalian cells, the phosphorylation of purine deoxyribonucleosides is mediated predominantly by 2 deoxyribonucleoside kinases: cytosolic deoxycytidine kinase (DCK) and mitochondrial deoxyguanosine kinase (DGUOK also known as DGK). DGUOK expression is ubiquitous, with highest levels in muscle, brain, liver and lymphoid tissues. Defects in DGUOK are a cause of mitochondrial DNA depletion syndrome (MDS). MDS is a clinically heterogeneous group of disorders characterized by a reduction in mitochondrial DNA (mtDNA) copy number. Primary mtDNA depletion is inherited as an autosomal recessive trait and may affect single organs, typically muscle or liver, or multiple tissues. Mitochondrial DNA depletion syndromes are phenotypically heterogeneous, autosomal recessive disorders characterized by tissue-specific reduction in mtDNA copy number. Affected individuals with the hepatocerebral form of mtDNA depletion syndrome have early progressive liver failure and neurologic abnormalities, hypoglycemia, and increased lactate in body fluids.Synonyms: DGK, DGUOK

Gene ID: 1716, 9606

UniProt: Q16854

Pathways: Ribonucleoside Biosynthetic Process