CERK antibody (C-Term)

Catalog No. ABIN359190

The Rabbit Polyclonal anti-CERK antibody (ABIN359190) specifically detects CERK in WB and EIA. The antibody is reactive with Human and Mouse samples.

Quick Overview for CERK antibody (C-Term) (ABIN359190)

Target: CERK (Ceramide Kinase (CERK))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CERK antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-CERK Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to CERK.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human CERK.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for CERK

Target: CERK (Ceramide Kinase (CERK))

Alternative Name: Ceramide Kinase

Background: Ceramide kinases convert the sphingolipid metabolite ceramide into ceramide-1-phosphate, both key mediators of cellular apoptosis and survival. Ceramide metabolism plays an essential role in the viability of neuronal cells, the membranes of which are particularly rich in sphingolipids. CERK catalyzes specifically the phosphorylation of ceramide to form ceramide 1-phosphate. This enzyme acts efficiently on natural and analog ceramides (C6, C8, C16 ceramides, and C8 dihydroceramide), and to a lesser extent on C2-ceramide and C6-dihydroceramide, but not on other lipids, such as various sphingosines. High level expression is noted in heart, brain, skeletal muscle, kidney and liver, moderate expression in peripheral blood leukocytes and thymus, and low expression in spleen, small intestine, placenta and lung.Synonyms: Acylsphingosine kinase, KIAA1646, LK4, Lipid kinase 4, hCERK

Gene ID: 64781, 9606

UniProt: Q8TCT0