MARK4 antibody (C-Term)

Catalog No. ABIN359301

The Rabbit Polyclonal anti-MARK4 antibody (ABIN359301) specifically detects MARK4 in WB and EIA. The antibody is reactive with Human samples.

Quick Overview for MARK4 antibody (C-Term) (ABIN359301)

Target: MARK4 (MAP/microtubule Affinity-Regulating Kinase 4 (MARK4))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MARK4 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-MARK4 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to MARK4.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human MARK4.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for MARK4

Target: MARK4 (MAP/microtubule Affinity-Regulating Kinase 4 (MARK4))

Alternative Name: MARK4

Background: MARK4 contains an N-terminal serine/threonine kinase domain, a central ubiquitin-associated domain, and a C-terminal KA1-associated kinase domain. RT-PCR analysis detects upregulated expression of the gene for MARK4 in nearly all clinical hepatocellular carcinoma cells. Northern blot analysis reveals ubiquitous expression of a 3.6-kb transcript, with highest expression in testis. Immunofluorescence microscopy demonstrates homogeneous cytoplasmic expression. Colony-forming assays show that MARK4 antisense reduces the growth of SNU475 cells. It has been suggested that MARK4 provides a growth advantage to cells.Synonyms: MAP microtubule affinity-regulating kinase 4, MARKL1

Gene ID: 57787

NCBI Accession: NP_001186796

UniProt: Q96L34