PIM3
Reactivity: Human
ELISA
Host: Rabbit
Polyclonal
Biotin
Application Notes
ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user.
Protocol
1. Mikkers, H. , et al. , Mol. Cell. Biol. 24(13): 6104-6115 (2004). 2. Eichmann, A. , et al. , Oncogene 19(9): 1215-1224 (2000).
Restrictions
For Research Use only
Format
Liquid
Concentration
0.25 mg/mL
Buffer
PBS with 0.09 % (W/V) sodium azide
Preservative
Sodium azide
Precaution of Use
This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Handling Advice
Avoid repeated freezing and thawing.
Storage
4 °C/-20 °C
Storage Comment
Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.
PIM3 is a memberof the Ser/Thr protein kinase family that may be involved in cell cycle progression and anti-apoptosis process. This protein has been implicated in proliferation of human hepatoma cell lines. PIM3 is widely expressed, with the exception of no expression observed in colon, thymus, and small intestine. PIM3 is expressed in human hepatoma cell lines but not in normal liver tissues.Synonyms: PIM-3, Serine/threonine-protein kinase pim-3