PIM3 antibody (C-Term)

Catalog No. ABIN359340

The Rabbit Polyclonal anti-PIM3 antibody (ABIN359340) specifically detects PIM3 in WB, IHC (p) and EIA. The antibody is reactive with Human samples.

Quick Overview for PIM3 antibody (C-Term) (ABIN359340)

Target: PIM3 (Pim-3 Oncogene (PIM3))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-PIM3 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to PIM3.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human PIM3.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Protocol: 1. Mikkers, H. , et al. , Mol. Cell. Biol. 24(13): 6104-6115 (2004). 2. Eichmann, A. , et al. , Oncogene 19(9): 1215-1224 (2000).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PIM3

Target: PIM3 (Pim-3 Oncogene (PIM3))

Alternative Name: PIM3

Background: PIM3 is a memberof the Ser/Thr protein kinase family that may be involved in cell cycle progression and anti-apoptosis process. This protein has been implicated in proliferation of human hepatoma cell lines. PIM3 is widely expressed, with the exception of no expression observed in colon, thymus, and small intestine. PIM3 is expressed in human hepatoma cell lines but not in normal liver tissues.Synonyms: PIM-3, Serine/threonine-protein kinase pim-3

Gene ID: 415116, 9606

UniProt: Q86V86

Pathways: Negative Regulation of Hormone Secretion