PIM3 antibody (AA 259-288)

Catalog No. ABIN6741524

This Rabbit Polyclonal antibody specifically detects PIM3 in WB and IHC (p). It exhibits reactivity toward Human and Mouse.

Quick Overview for PIM3 antibody (AA 259-288) (ABIN6741524)

Target: PIM3 (Pim-3 Oncogene (PIM3))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Product Details anti-PIM3 Antibody

Binding Specificity: AA 259-288

Specificity: This PIM3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 259-288 amino acids from the C-terminal region of human PIM3.

Purification: Ammonium sulfate precipitation

Application Details

Application Notes: Approved: IHC-P (10 μg/mL), WB (1:1000)

Usage: Immunohistochemistry: This antibody was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for this antibody was determined to be 10 μg/mL. Please note that cross-reactivity with mouse has been confirmed in WB only. The antibody has not been evaluated for use in IHC with mouse samples at this time.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: PBS, 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C.

Expiry Date: 6 months

Target Details for PIM3

Target: PIM3 (Pim-3 Oncogene (PIM3))

Alternative Name: PIM3 / PIM-3

Background: Name/Gene ID: PIM3
Subfamily: PIM
Family: Protein Kinase

Synonyms: PIM3, KID1, Pim-3 oncogene, Serine/threonine kinase Pim-3, Pim-3

Gene ID: 415116

UniProt: Q86V86

Pathways: Negative Regulation of Hormone Secretion