MAP3K12 antibody (C-Term)

Catalog No. ABIN359408

The Rabbit Polyclonal anti-MAP3K12 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect MAP3K12 in samples from Human and Mouse.

Quick Overview for MAP3K12 antibody (C-Term) (ABIN359408)

Target: MAP3K12 (Mitogen-Activated Protein Kinase Kinase Kinase 12 (MAP3K12))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAP3K12 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-MAP3K12 Antibody

Binding Specificity: C-Term

Specificity: This antibody detects MAP3K12 at C-term.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human ZPK.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 100 - 1: 500. Immunohistochemistry 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for MAP3K12

Target: MAP3K12 (Mitogen-Activated Protein Kinase Kinase Kinase 12 (MAP3K12))

Alternative Name: MAP3K12

Background: ZPK is a member of serine/threonine protein kinase family. This kinase contains a leucine-zipper domain, and is predominately expressed in neuronal cells. The phosphorylation state of this kinase in synaptic terminals was shown to be regulated by membrane depolarization via calcineurin. This kinase forms heterodimers with leucine zipper containing transcription factors, such as cAMP responsive element binding protein (CREB) and MYC, and thus may play a regulatory role in PKA or retinoic acid induced neuronal differentiation.Synonyms: Dual leucine zipper bearing kinase, Leucine-zipper protein kinase, MAPK-upstream kinase, Mitogen-activated protein kinase kinase kinase 12, Mixed lineage kinase

Molecular Weight: 93188 Da

Gene ID: 7786, 9606

UniProt: Q12852

Pathways: MAPK Signaling