MAPK14 antibody (Tyr322)

Catalog No. ABIN359422

This Rabbit Polyclonal antibody specifically detects MAPK14 in WB and EIA. It exhibits reactivity toward Human.

Quick Overview for MAPK14 antibody (Tyr322) (ABIN359422)

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPK14 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-MAPK14 Antibody

Binding Specificity: Tyr322

Specificity: This antibody detects MAPK14 (p38).

Purification: Protein A column, followed by peptide affinity purification.

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y322 of human MAPK14.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA 1: 1,000. Western blot 1: 50 - 1: 100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody at 2 - 8 °C up to one month or (in aliquots) at -20 °C for longer.

Target Details for MAPK14

Target: MAPK14 (Mitogen-Activated Protein Kinase 14 (MAPK14))

Alternative Name: MAP Kinase p38 alpha / MAPK14

Background: MAPK14 is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various environmental stresses and proinflammatory cytokines. The activation requires its phosphorylation by MAP kinase kinases (MKKs), or its autophosphorylation triggered by the interaction of MAP3K7IP1/TAB1 protein with this kinase. The substrates of this kinase include transcription regulator ATF2, MEF2C, and MAX, cell cycle regulator CDC25B, and tumor suppressor p53, which suggest the roles of this kinase in stress related transcription and cell cycle regulation, as well as in genotoxic stress response.Synonyms: CSBP, CSBP1, CSBP2, CSPB1, Cytokine suppressive anti-inflammatory drug-binding protein, MAX-interacting protein 2, MXI2, Mitogen-activated protein kinase 14, SAPK2A, p38 alpha

Molecular Weight: 41293 Da

Gene ID: 1432, 9606

UniProt: Q16539

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Cellular Response to Molecule of Bacterial Origin, Regulation of Muscle Cell Differentiation, Regulation of Cell Size, Hepatitis C, Toll-Like Receptors Cascades, Autophagy, Thromboxane A2 Receptor Signaling, BCR Signaling, S100 Proteins