EPH Receptor B2 antibody (C-Term)

Catalog No. ABIN359813

The Rabbit Polyclonal anti-EPH Receptor B2 antibody has been validated for WB, IHC (p) and EIA. It is suitable to detect EPH Receptor B2 in samples from Human.

Quick Overview for EPH Receptor B2 antibody (C-Term) (ABIN359813)

Target: EPH Receptor B2 (EPHB2)

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This EPH Receptor B2 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-EPH Receptor B2 Antibody

Binding Specificity: C-Term

Specificity: This antibody reacts to EphB2.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the C-terminal region of human EphB2.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/10 - 1/50.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for EPH Receptor B2

Target: EPH Receptor B2 (EPHB2)

Alternative Name: EPHB2

Background: Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into 2 groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. The ligand-activated form of EphB2, which belongs to the Tyr family of protein kinases, interacts with multiple proteins, including GTPase-activating protein (RASGAP) through its SH2 domain. It binds RASGAP through the juxtamembrane tyrosines residues, and also interacts with PRKCABP and GRIP1 This type I membrane protein is expressed in brain, heart, lung, kidney, placenta, pancreas, liver and skeletal muscle. It is preferentially expressed in fetal brain. This protein contains putatively 2 fibronectin type III domains and 1 sterile alpha motif (SAM) domain.Synonyms: DRT, DRT, EPHT3, EPTH3, ERK, Ephrin type-B receptor 2, HEK5, NY-REN-47, Receptor protein-tyrosine kinase HEK5, Renal carcinoma antigen NY-REN-47, TYRO5, Tyrosine-protein kinase TYRO5, Tyrosine-protein kinase receptor EPH-3

Gene ID: 2048, 9606

UniProt: P29323

Pathways: RTK Signaling, Regulation of long-term Neuronal Synaptic Plasticity, S100 Proteins