PIK3R2 antibody (Tyr464)

Catalog No. ABIN360467

The Rabbit Polyclonal anti-PIK3R2 antibody is suitable to detect PIK3R2 in samples from Human. It has been validated for WB and EIA.

Quick Overview for PIK3R2 antibody (Tyr464) (ABIN360467)

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PIK3R2 antibody is un-conjugated

Application: Western Blotting (WB), Enzyme Immunoassay (EIA)

Product Details anti-PIK3R2 Antibody

Binding Specificity: Tyr464

Specificity: This antibody reacts to PIK3R2.

Purification: Protein A column, followed by peptide affinity purification

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide corresponding to amino acid residues surrounding Y464 of human PIK3R2.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for PIK3R2

Target: PIK3R2 (PI3K p85b) (Phosphoinositide 3 Kinase, p85 beta (PI3K p85b))

Alternative Name: PIK3R2

Background: Protein kinases are enzymes that transfer a phosphate group from a phosphate donor, generally the g phosphate of ATP, onto an acceptor amino acid in a substrate protein. By this basic mechanism, protein kinases mediate most of the signal transduction in eukaryotic cells, regulating cellular metabolism, transcription, cell cycle progression, cytoskeletal rearrangement and cell movement, apoptosis, and differentiation. The family has been classified into 8 major groups based on sequence comparison of their tyrosine (PTK) or serine/threonine (STK) kinase catalytic domains.PIK3R2 binds to activated Protein Tyrosine Kinases, which are phosphorylated, through its SH2 domain, and acts as an adaptor, mediating the association of the P110 catalytic unit to the plasma membrane.Synonyms: PI 3 Kinase p85 beta, PI3-kinase regulatory subunit beta, PI3K regulatory subunit beta, Phosphatidylinositol 3-kinase 85 kDa regulatory subunit beta, Phosphatidylinositol 3-kinase regulatory subunit beta

Gene ID: 5296, 9606

UniProt: O00459

Pathways: VEGF Signaling, BCR Signaling