WEE1 antibody (Middle Region)

Catalog No. ABIN360580

This Rabbit Polyclonal antibody specifically detects WEE1 in WB, IHC (p) and EIA. It exhibits reactivity toward Human and Mouse.

Quick Overview for WEE1 antibody (Middle Region) (ABIN360580)

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Reactivity: Human, Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This WEE1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Enzyme Immunoassay (EIA)

Product Details anti-WEE1 Antibody

Binding Specificity: Middle Region

Specificity: This antibody reacts to WEE1.

Purification: Protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS

Immunogen: This antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide selected from the center region of human WEE1.

Isotype: Ig Fraction

Application Details

Application Notes: ELISA: 1/1,000. Western blotting: 1/100 - 1/500. Immunohistochemistry: 1/50 - 1/100.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 0.25 mg/mL

Buffer: PBS with 0.09 % (W/V) sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Store the antibody undiluted at 2-8 °C for one month or (in aliquots) at-20 °C for longer.

Target Details for WEE1

Target: WEE1 (WEE1 Homolog (S. Pombe) (WEE1))

Alternative Name: WEE1 / Wee1-Like Protein Kinase

Background: WEE1 is a nuclear protein, which is a tyrosine kinase belonging to the Ser/Thr family of protein kinases. This protein catalyzes the inhibitory tyrosine phosphorylation of CDC2/cyclin B kinase, and appears to coordinate the transition between DNA replication and mitosis by protecting the nucleus from cytoplasmically activated CDC2 kinase.Synonyms: WEE1hu, Wee1A kinase

Gene ID: 7465, 9606

UniProt: P30291

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases, M Phase