GAS6 antibody

Catalog No. ABIN360889

This Rat Monoclonal antibody specifically detects GAS6 in WB. It exhibits reactivity toward Human.

Quick Overview for GAS6 antibody (ABIN360889)

Target: GAS6 (Growth Arrest-Specific 6 (GAS6))

Reactivity: Human

Host: Rat

Clonality: Monoclonal

Conjugate: This GAS6 antibody is un-conjugated

Application: Western Blotting (WB)

Clone: 6J2

Product Details anti-GAS6 Antibody

Specificity: The monoclonal antibody detects Gas6.

Purification: Protein A/G Affinity Chromatography

Immunogen: Purified Mouse Recombinant Gas6

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.

Buffer: 0.2 μm filtered PBS without preservatives or stabilizers

Preservative: Without preservative

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for GAS6

Target: GAS6 (Growth Arrest-Specific 6 (GAS6))

Alternative Name: GAS6 / AXLLG

Background: Gas6 (Growth Arrest Specific 6) is one of six structurally unrelated proteins that are secreted by a wide variety of cell types in response to growth arrest. Gas6 and the structurally related Protein S are vitamin K-dependent and have an extensively gamma-carboxylated N-terminal Gla domain, four EGF like repeats, and a C-terminal region with homology to steroid hormone binding globulin (SHBG). Human Gas6 is a 75 kDa protein that shares 77 % -79 % aa sequence identity with mouse and rat Gas6. Alternate splicing generates isoforms that lack the Gla domain and/or the spacer between the EGF-like and SHBG regions. Gas6 binds and induces signaling through the receptor tyrosine kinases Axl, Dtk, and Mer. Human Gas6 interacts with both mouse and rat orthologs of these receptors. The full length isoform may be cleaved, resulting in release of the free SHBG region which can independently activate Axl. Shed soluble forms of Axl and Mer bind Gas6 and function as decoy receptors.Synonyms: AXL receptor tyrosine kinase ligand, GAS-6, Growth arrest-specific protein 6

Gene ID: 2621

NCBI Accession: NP_000811

UniProt: Q14393

Pathways: RTK Signaling, Carbohydrate Homeostasis, Production of Molecular Mediator of Immune Response