Sonic Hedgehog antibody (C-Term)

Catalog No. ABIN360935

The Rat Monoclonal anti-Sonic Hedgehog antibody is suitable to detect Sonic Hedgehog in samples from Mouse. It has been validated for WB and IHC (fro).

Quick Overview for Sonic Hedgehog antibody (C-Term) (ABIN360935)

Target: Sonic Hedgehog (SHH)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Sonic Hedgehog antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: 4A37

Product Details anti-Sonic Hedgehog Antibody

Binding Specificity: C-Term

Specificity: This antibody detects Shh, it does not cross react with dhh or ihh.

Purification: Protein A/G affinity chromatography

Immunogen: Recombinant Sonic Hedgehog (Shh) C-Terminal fragment

Isotype: IgG2

Application Details

Application Notes: Western Blot: 1/500 - 1/1000.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore with 200 μL sterile PBS and the final concentration is 500 μg/mL.

Handling Advice: Avoid repeated freezing and thawing.

Storage: 4 °C/-20 °C

Storage Comment: Prior to reconstitution store at 2-8 °C. Following reconstitution store undiluted at 2-8 °C for one month or (in aliquots) at -20 °C for longer.

Target Details for Sonic Hedgehog

Target: Sonic Hedgehog (SHH)

Background: Synonyms: HHG-1, Sonic hedgehog protein

Gene ID: 20423

NCBI Accession: NP_033196

UniProt: Q62226

Pathways: Hedgehog Signaling, Dopaminergic Neurogenesis, Regulation of Muscle Cell Differentiation, Tube Formation, Skeletal Muscle Fiber Development