Endoglin antibody (Biotin)

Catalog No. ABIN360953

The Rat Monoclonal anti-Endoglin antibody has been validated for WB, FACS, IP and IHC (fro). It is suitable to detect Endoglin in samples from Mouse.

Quick Overview for Endoglin antibody (Biotin) (ABIN360953)

Target: Endoglin (ENG)

Reactivity: Mouse

Host: Rat

Clonality: Monoclonal

Conjugate: This Endoglin antibody is conjugated to Biotin

Application: Western Blotting (WB), Flow Cytometry (FACS), Immunoprecipitation (IP), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: MJ7-18

Product Details anti-Endoglin Antibody (Biotin)

Purification: Protein G Chromatography

Immunogen: Inflamed mouse skin

Isotype: IgG2a

Application Details

Application Notes: ELISA. Western Blot: 1-2 μg/mL. Immuoprecipitation: 1-2 μg. FACS analysis: 3-20 μg/mL. Immunohistochemistry on frozen sections.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Handling

Reconstitution: Restore in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS, 0.02 % Sodium Azide, 50xBSA

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: This product is photosensitive and should be protected from light

Storage: 4 °C

Storage Comment: Prior to and following reconstitution store the antibody undiluted at 2-8 °C. DO NOT FREEZE!

Target Details for Endoglin

Target: Endoglin (ENG)

Alternative Name: CD105 / Endoglin

Background: Mouse Endoglin is a disulfide-linked homodimeric protein. Based on N-terminal sequence analysis the primary structure of recombinant mature Endoglin starts at Glu 26. Endoglin has a calculated monomeric molecular mass of 61 kDa but as a result of glycosylation, migrates at approximately 70-75 kDa under reducing conditions in SDS-PAGE. Endoglin, also known as CD105, is a Type I integral membrane glycoprotein with a large, disulfide-linked, extracellular region and a short, constitutively phosphorylated, cytoplasmic tail. Two splice variants of human Endoglin, the S-Endoglin and L-Endoglin that differ in the length of their cytoplasmic tails have been identified. Endoglin is highly expressed on vascular endothelial cells, chondrocytes, and syncytiotrophoblasts of term placenta. It is also found on activated monocytes, bone marrow pro-erythroblasts, and leukemic cells of lymphoid and myeloid lineages. Human and mouse Endoglin share approximately 70 % and 97 % amino acid sequence identity in their extracellular and intracellular domains, respectively. Endoglin binds TGF-ß1 and TGF-ß3 but not TGF-ß2 efficiently by associating with TGF-ß type II receptor (TßRII). Endoglin has been shown to be a powerful marker of neovascularization. It is also useful as a functional marker that defines long-term repopulating hematopoietic stem cells.Synonyms: END, ENG, HHT1, ORW, ORW1

Gene ID: 10090

UniProt: Q63961