NEFM antibody (C-Term)

Catalog No. ABIN361355

The Chicken Polyclonal anti-NEFM antibody has been validated for WB and IF. It is suitable to detect NEFM in samples from Rat. There is 1 publication available.

Quick Overview for NEFM antibody (C-Term) (ABIN361355)

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Reactivity: Rat

Host: Chicken

Clonality: Polyclonal

Conjugate: This NEFM antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-NEFM Antibody

Binding Specificity: C-Term

Specificity: Specific for the ~145k Neurofilament M protein.

Cross-Reactivity: Chicken, Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Purification: Total IgY fraction

Immunogen: Preparation containing the extreme C-terminus expressed in and purified from E. Coli

Application Details

Application Notes: Recommended Dilution: WB: 1:5,000 IF: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: total IgY fraction in PBS + 10 mM Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

References for anti-NEFM antibody (ABIN361355)

Glass, Kelm-Nelson, Szot, Lake, Connor, Ciucci: "Functional characterization of extrinsic tongue muscles in the Pink1-/- rat model of Parkinson disease." in: PloS one, Vol. 15, Issue 10, pp. e0240366, (2020) (PubMed).

Target Details for NEFM

Target: NEFM (Neurofilament Triplet M Protein (NEFM))

Alternative Name: NEFM

Background: Neurofilaments are the 10nm or intermediate filament proteins found specifically in neurons, and are composed predominantly of three major proteins called NF-L, NF-M and NF-H (1). NF-M is the neurofilament middle or medium molecular weight polypeptide and runs on SDS-PAGE gels at 145-160 kDa, with some variability across species boundaries. Antibodies to NF-M are useful for identifying neuronal cells and their processes in tissue sections and in tissue culture. NF-M antibodies can also be useful to visualize neurofilament accumulations seen in many neurological diseases, such as Amyotrophic Lateral Sclerosis (Lou Gehrig's disease) and Alzheimer's disease (2). Anti-Neurofilament M Western blot of rat cortex lysate showing specific immunolableing of the ~ 145k NF-M protein.

Molecular Weight: '145 kDa

Gene ID: 24588

UniProt: P12839

Pathways: Brown Fat Cell Differentiation