UCHL1 antibody

Catalog No. ABIN361370

The Chicken Polyclonal anti-UCHL1 antibody has been validated for WB and IF. It is suitable to detect UCHL1 in samples from Human.

Quick Overview for UCHL1 antibody (ABIN361370)

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This UCHL1 antibody is un-conjugated

Application: Western Blotting (WB), Immunofluorescence (IF)

Product Details anti-UCHL1 Antibody

Specificity: Specific for the ~24kDa UCHL1 protein. It is expected that it will work on other mammalian tissues.

Cross-Reactivity: Cow (Bovine), Human, Mouse (Murine), Rat (Rattus)

Predicted Reactivity: most mammalian

Purification: Total IgY fraction

Immunogen: Recombinant full length human UCHL1 purified from E. coli

Application Details

Application Notes: Recommended Dilution: WB: 1:2,500 IF: 1:500 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: total IgY fraction in PBS + 10 mM Sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Target Details for UCHL1

Target: UCHL1 (Ubiquitin Carboxyl-terminal Esterase L1 (Ubiquitin Thiolesterase) (UCHL1))

Alternative Name: UCHL1

Background: Ubiquitin C-terminal hydrolase 1 (UCHL1) is also known as ubiquitin carboxyl esterase L1, ubiquitin thiolesterase, neuron-specific protein PGP9.5 and Park5. It was originally identified as a major component of the neuronal cytoplasm from 2-dimensional gel analysis of brain tissues, and was given the name PGP9.5 (1). It was later found that ubiquitin C-terminal hydrolase enzyme activity was associated with the PGP9.5 protein (2). The ubiquitin C-terminal hydrolases cleave ubiquitin from other molecules. Regulation of the ubiquitin pathway is very important and many disease states are associated with defects in this pathway. Genetic knockout of UCHL1 in mice results in a motor neuron degeneration similar to the spontaneous gracile axonal dystrophy (gad) mutant mice (3). Point mutations in the UCHL1 gene are associated with some forms of human Parkinson's disease (4). Since UCHL1 is heavily expressed in neurons, it is released in large amounts following injury or degeneration, so the detection of UCHL1 in CSF and other bodily fluids can be used as a biomarker. Anti-UCHL1

Molecular Weight: '24 kDa

Gene ID: 7365

UniProt: P09936

Pathways: Feeding Behaviour