Fibrillarin antibody

Catalog No. ABIN361375

This Mouse Monoclonal antibody specifically detects Fibrillarin in WB, IHC, ELISA and ICC. It exhibits reactivity toward Saccharomyces cerevisiae and has been mentioned in 2+ publications.

Quick Overview for Fibrillarin antibody (ABIN361375)

Target: Fibrillarin (FBL)

Reactivity: Saccharomyces cerevisiae

Host: Mouse

Clonality: Monoclonal

Conjugate: This Fibrillarin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone: 38F3

Product Details anti-Fibrillarin Antibody

Specificity: Specific for endogenous levels of the ~34 kDa Fibrillarin /Nop1p protein.

Cross-Reactivity: Chicken, Cow, Drosophila melanogaster, Human, Mouse, Pig, Rat

Characteristics: Our Anti-Fibrillarin Antibody primary antibody is mouse monoclonal. It detects Fibrillarin and is protein g purified. It is great for use in ELISA, WB, IHC, ICC.

Purification: Protein G purified culture supernatant.

Immunogen: Yeast nuclear preparation

Isotype: IgG1

Application Details

Application Notes:

Dilution Range: ICC: 1:100-1:500

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS + 50 % glycerol and 5 mm Sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-Fibrillarin antibody (ABIN361375)

Vendramin, Verheyden, Ishikawa, Goedert, Nicolas, Saraf, Armaos, Delli Ponti, Izumikawa, Mestdagh, Lafontaine, Tartaglia, Takahashi, Marine, Leucci: "SAMMSON fosters cancer cell fitness by concertedly enhancing mitochondrial and cytosolic translation." in: Nature structural & molecular biology, Vol. 25, Issue 11, pp. 1035-1046, (2019) (PubMed).

Langhendries, Nicolas, Doumont, Goldman, Lafontaine: "The human box C/D snoRNAs U3 and U8 are required for pre-rRNA processing and tumorigenesis." in: Oncotarget, Vol. 7, Issue 37, pp. 59519-59534, (2018) (PubMed).

Target Details for Fibrillarin

Target: Fibrillarin (FBL)

Alternative Name: Fibrillarin (Nop1p)

Background:

Synonyms: 34 kD nucleolar scleroderma antigen antibody, 34 kDa nucleolar scleroderma antigen antibody, Fbl antibody, FBRL_HUMAN antibody, FIB antibody, FIB1 antibody, FLRN antibody, Histone-glutamine methyltransferase antibody, Nop1p antibody, RNA U3 small nucleolar interacting protein 1 antibody, RNU3IP1 antibody, rRNA 2' O methyltransferase fibrillarin antibody, rRNA 2'-O-methyltransferase fibrillarin antibody

Description Logic: Our Anti-Fibrillarin Antibody primary antibody is mouse monoclonal. It detects Fibrillarin and is protein g purified. It is great for use in ELISA, WB, IHC, ICC.

Gene Name Alternatives: NOP1

Molecular Weight: 34 kDa

Gene ID: 851548

UniProt: P15646

Pathways: Ribonucleoside Biosynthetic Process