GRIN1/NMDAR1 antibody (AA 1-564)

Catalog No. ABIN361405

This Mouse Monoclonal antibody specifically detects GRIN1/NMDAR1 in WB, IHC, ELISA and ICC. It exhibits reactivity toward Rat. It has been mentioned in 4+ publications

Quick Overview for GRIN1/NMDAR1 antibody (AA 1-564) (ABIN361405)

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Reactivity: Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This GRIN1/NMDAR1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunocytochemistry (ICC)

Clone: R1JHL

Product Details anti-GRIN1/NMDAR1 Antibody

Binding Specificity: AA 1-564

Specificity: Specific for endogenous levels of the ~120 kDa NR1 subunit of the NMDA receptor.

Cross-Reactivity: Human, Mouse, Rat

Characteristics: Our Anti-NMDA NR1 Subunit Antibody primary antibody is mouse monoclonal. It detects human, mouse, and rat NMDA NR1 Subunit and is culture supernatant. It is great for use in ELISA, WB, IHC, ICC.

Purification: Culture supernatant.

Immunogen: Fusion protein containing amino acids 1-564 of the NR1 subunit of rat NMDA receptor.

Isotype: IgG

Application Details

Application Notes:

Dilution Range: IP: 3 μg per 200 μg lysate

Dilution Range: ICC: 1:10

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Lyophilized

Buffer: Lyophilized

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Reconstitute in 50 μl phosphate buffered saline (PBS: 137 mM NaCl, 7.5 mM Na2HPO4, 2.7 mM KCl, 1.5 mM KH2PO4, pH 7.4) before use. After reconstitution it is recommended that the undiluted antibody be aliquoted into smaller working volumes (10-30 uL/vial depending on usage) and stored long term at -20° C or -80° C, while keeping a working aliquot stored at 4° C for short term. Avoid freeze/thaw cycles. Stable for at least 1 year.

Expiry Date: 12 months

References for anti-GRIN1/NMDAR1 antibody (ABIN361405)

Quillinan, Grewal, Deng, Shimizu, Yonchek, Strnad, Traystman, Herson: "Region-specific role for GluN2B-containing NMDA receptors in injury to Purkinje cells and CA1 neurons following global cerebral ischemia." in: Neuroscience, Vol. 284, pp. 555-65, (2014) (PubMed).

Hicklin, Wu, Radcliffe, Freund, Goebel-Goody, Correa, Proctor, Lombroso, Browning: "Alcohol inhibition of the NMDA receptor function, long-term potentiation, and fear learning requires striatal-enriched protein tyrosine phosphatase." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 108, Issue 16, pp. 6650-5, (2011) (PubMed).

Davies, Goebel-Goody, Coultrap, Browning: "Long term synaptic depression that is associated with GluR1 dephosphorylation but not alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionic acid (AMPA) receptor internalization." in: The Journal of biological chemistry, Vol. 283, Issue 48, pp. 33138-46, (2008) (PubMed).

Zhang, Dong, Zhang, Ichinose, Wu, Culley, Crosby, Tanzi, Xie: "Isoflurane-induced caspase-3 activation is dependent on cytosolic calcium and can be attenuated by memantine." in: The Journal of neuroscience : the official journal of the Society for Neuroscience, Vol. 28, Issue 17, pp. 4551-60, (2008) (PubMed).

Target Details for GRIN1/NMDAR1

Target: GRIN1/NMDAR1 (GRIN1) (Glutamate Receptor, Ionotropic, N-Methyl D-Aspartate 1 (GRIN1))

Alternative Name: GRIN1

Background:

Synonyms: GluN1 antibody, Glutamate [NMDA] receptor subunit zeta-1 antibody, Glutamate receptor ionotropic N methyl D aspartate 1 antibody, Glutamate receptor ionotropic N-methyl-D aspartate subunit 1 antibody, glutamate receptor ionotropic NMDA 1 antibody, Grin1 antibody, MRD8 antibody, N methyl D aspartate receptor antibody, N methyl D aspartate receptor channel subunit zeta 1 antibody, N methyl D aspartate receptor subunit NR1 antibody, N-methyl-D-aspartate receptor subunit NR1 antibody, NMD-R1 antibody, NMDA 1 antibody, NMDA R1 antibody, NMDAR1 antibody, NMDA receptor 1 antibody, NMDA1 antibody, NMDAR antibody, NMDZ1_HUMAN antibody, NR1 antibody

Description Logic: Our Anti-NMDA NR1 Subunit Antibody primary antibody is mouse monoclonal. It detects human, mouse, and rat NMDA NR1 Subunit and is culture supernatant. It is great for use in ELISA, WB, IHC, ICC.

Gene Name Alternatives: GRIN1

Molecular Weight: 120 kDa

Gene ID: 24408

UniProt: P35439

Pathways: Synaptic Membrane, Feeding Behaviour, Regulation of long-term Neuronal Synaptic Plasticity