Tyrosine Hydroxylase antibody

Catalog No. ABIN361414

This Sheep Polyclonal antibody specifically detects Tyrosine Hydroxylase in WB, IHC and IF. It exhibits reactivity toward Rat.

Quick Overview for Tyrosine Hydroxylase antibody (ABIN361414)

Target: Tyrosine Hydroxylase (TH)

Reactivity: Rat

Host: Sheep

Clonality: Polyclonal

Conjugate: This Tyrosine Hydroxylase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Tyrosine Hydroxylase Antibody

Specificity: Specific for the ~60k tyrosine hydroxylase protein.

Cross-Reactivity: Mammalian

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: SDS-denatured, native rat tyrosine hydroxylase purified from pheochromocytoma

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 IF (frozen sections, unpublished observations): 1:1000 IHC (frozen sections, unpublished observations): 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Target Details for Tyrosine Hydroxylase

Target: Tyrosine Hydroxylase (TH)

Alternative Name: Tyrosine Hydroxylase

Background: Tyrosine hydroxylase (TH) is the rate-limiting enzyme in the synthesis of the catecholamines dopamine and norepinephrine. TH antibodies can therefore be used as markers for dopaminergic and noradrenergic neurons in a variety of applications including depression, schizophrenia, Parkinson?s disease and drug abuse (Kish et al., 2001, Zhu et al., 2000, Zhu et al., 1999). TH antibodies can also be used to explore basic mechanisms of dopamine and norepinephrine signaling (Witkovsky et al., 2000, Salvatore et al., 2001). Anti-Tyrosine Hydroxylase Western blot of 10 ug of rat caudate lysate showing specific immunolabeling of the ~60k tyrosine hydroxylase protein.

Molecular Weight: '60 kDa

Gene ID: 25085

UniProt: P04177

Pathways: Dopaminergic Neurogenesis, Response to Water Deprivation, Sensory Perception of Sound, Carbohydrate Homeostasis, Feeding Behaviour