MAPT antibody (pSer416)

Catalog No. ABIN361456

This Rabbit Polyclonal antibody specifically detects MAPT in WB, ELISA and IHC. It exhibits reactivity toward Ratand has been mentioned in 1 publication.

Quick Overview for MAPT antibody (pSer416) (ABIN361456)

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPT antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-MAPT Antibody

Binding Specificity: pSer416

Specificity: Specific for ~59, 65, 68 kDa tau protein phosphorylated at Ser416. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: bovine, canine, human, non-human primate

Characteristics: Our Tau (Ser416) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Tau (Ser416) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser416 of rat tau, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-MAPT antibody (ABIN361456)

Wang, Hall, Lee, Kamgar-Parsi, Bi, Baudry: "The tyrosine phosphatase PTPN13/FAP-1 links calpain-2, TBI and tau tyrosine phosphorylation." in: Scientific reports, Vol. 7, Issue 1, pp. 11771, (2019) (PubMed).

Target Details for MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Alternative Name: MAPT

Background:

Synonyms: AI413597 antibody, AW045860 antibody, DDPAC antibody, FLJ31424 antibody, FTDP 17 antibody, G protein beta1/gamma2 subunit interacting factor 1 antibody, MAPT antibody, MAPTL antibody, MGC134287 antibody, MGC138549 antibody, MGC156663 antibody, Microtubule associated protein tau antibody, Microtubule associated protein tau isoform 4 antibody, Microtubule-associated protein tau antibody, MSTD antibody, Mtapt antibody, MTBT1 antibody, MTBT2 antibody, Neurofibrillary tangle protein antibody, Paired helical filament tau antibody, Paired helical filament-tau antibody, PHF tau antibody, PHF-tau antibody, PPND antibody, PPP1R103 antibody, Protein phosphatase 1 regulatory subunit 103 antibody, pTau antibody, RNPTAU antibody, TAU antibody, TAU_HUMAN antibody, Tauopathy and respiratory failure included antibody

Description Logic: Our Tau (Ser416) rabbit polyclonal phosphospecific primary antibody is produced . It detects Tau (Ser416) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB, IHC.

Gene Name Alternatives: MAPT

Molecular Weight: 59 kDa/65 kDa/68 kDa

Gene ID: 69329

UniProt: P19332

Pathways: MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size