MAPT antibody (pSer416)

Catalog No. ABIN361456

This Rabbit Polyclonal antibody specifically detects MAPT in WB. It exhibits reactivity toward Ratand has been mentioned in 1 publication.

Quick Overview for MAPT antibody (pSer416) (ABIN361456)

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MAPT antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-MAPT Antibody

Binding Specificity: pSer416

Specificity: Specific for ~59, 65, 68k tau protein phosphorylated at Ser416.

Cross-Reactivity: Mouse (Murine), Rat (Rattus)

Predicted Reactivity: bovine, canine, human, non-human primate

Purification: Antigen Affinity Purified from Pooled Serum

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser416 conjugated to KLH

Application Details

Application Notes: Recommended Dilution: WB: 1:1000 Quality Control: Western blots performed on each lot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 100 μL in 10 mM HEPES (  pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

References for anti-MAPT antibody (ABIN361456)

Wang, Hall, Lee, Kamgar-Parsi, Bi, Baudry: "The tyrosine phosphatase PTPN13/FAP-1 links calpain-2, TBI and tau tyrosine phosphorylation." in: Scientific reports, Vol. 7, Issue 1, pp. 11771, (2019) (PubMed).

Target Details for MAPT

Target: MAPT (Microtubule-Associated Protein tau (MAPT))

Alternative Name: MAPT

Background: Tau is a key microtubule-associated protein that plays an important role in the formation of microtubules in axons (Binder et al. 1985). Six tau isoforms have been identified as products of a single gene produced by alternative mRNA splicing (Goedert 1990). Tau mutations have been implicated in many neurodegenerative disorders such as Alzheimer’s disease (AD), Pick’s disease and progressive supranuclear palsy. It has been well documented that hyperphosphorylated tau is a major component of paired helical filaments in AD brain (Lee 1995). Serine 416 has been demonstrated to be a major phosphorylation site in vitro by CaM kinase II (Steiner at al. 1990). Anti-Phospho-Ser416 Tau Western blot of rat brain homogenate showing specific immunolabeling of the ~59, 65, 68k Tau isoforms phosphorylated at Ser416(control). Immunolabeling is blocked by preadsorption with the phospho-peptide used as antigen (Peptide) but not by the corresponding dephospho-peptide (not shown).

Molecular Weight: 59/65/68 kDa

Gene ID: 69329

UniProt: P19332

Pathways: MAPK Signaling, Microtubule Dynamics, M Phase, Regulation of Cell Size