SYN1 antibody (pSer62, pSer67)

Catalog No. ABIN361494

This anti-SYN1 antibody is a Rabbit Polyclonal antibody detecting SYN1 in WB and ELISA. Suitable for Rat. This Primary Antibody has been cited in 3+ publications.

Quick Overview for SYN1 antibody (pSer62, pSer67) (ABIN361494)

Target: SYN1 (Synapsin I (SYN1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SYN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SYN1 Antibody

Binding Specificity: pSer62, pSer67

Specificity: Specific for endogenous levels of the ~78 kDa synapsin I doublet phosphorylated at Ser62,67. Immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide.

Cross-Reactivity: Human, Mouse, Rat

Predicted Reactivity: bovine, mouse

Characteristics: Our Synapsin (Ser62,67) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Synapsin (Ser62,67) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser62/67 of rat synapsin, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-SYN1 antibody (ABIN361494)

Chanaday, Vilcaes, de Paul, Torres, Degano, Roth: "Glutamate release machinery is altered in the frontal cortex of rats with experimental autoimmune encephalomyelitis." in: Molecular neurobiology, Vol. 51, Issue 3, pp. 1353-67, (2015) (PubMed).

Leal-Ortiz, Waites, Terry-Lorenzo, Zamorano, Gundelfinger, Garner: "Piccolo modulation of Synapsin1a dynamics regulates synaptic vesicle exocytosis." in: The Journal of cell biology, Vol. 181, Issue 5, pp. 831-46, (2008) (PubMed).

Tallent, Varghis, Skorobogatko, Hernandez-Cuebas, Whelan, Vocadlo, Vosseller: "In vivo modulation of O-GlcNAc levels regulates hippocampal synaptic plasticity through interplay with phosphorylation." in: The Journal of biological chemistry, Vol. 284, Issue 1, pp. 174-81, (2008) (PubMed).

Target Details for SYN1

Target: SYN1 (Synapsin I (SYN1))

Alternative Name: SYN1

Background:

Synonyms: Brain protein 4.1 antibody, SYN 1 antibody, SYN 1a antibody, SYN 1b antibody, SYN I antibody, SYN1 antibody, SYN1_HUMAN antibody, SYN1a antibody, SYN1b antibody, Synapsin 1 antibody, Synapsin I antibody, Synapsin-1 antibody, Synapsin1 antibody, SynapsinI antibody, SYNI antibody

Description Logic: Our Synapsin (Ser62,67) rabbit polyclonal phosphospecific primary antibody is produced . It detects Synapsin (Ser62,67) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: SYN1

Molecular Weight: 78 kDa

Gene ID: 281510

UniProt: P17599