IFNAR1 antibody (pSer535, pSer539)

Catalog No. ABIN361505

The Rabbit Polyclonal anti-IFNAR1 antibody has been validated for WB and ELISA. It is suitable to detect IFNAR1 in samples from Human. There are 2+ publications available.

Quick Overview for IFNAR1 antibody (pSer535, pSer539) (ABIN361505)

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This IFNAR1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-IFNAR1 Antibody

Binding Specificity: pSer535, pSer539

Specificity: Specific for IFNAR1 protein phosphorylated at Ser535,539. Note: the molecular weight of the IFNAR1 varies with cell line (different levels of glycosylation) in 293 and HeLa Cells, the mature form is ~110 kDa - 130 kDa.

Cross-Reactivity: Human

Predicted Reactivity: bovine, canine, mouse, non-human primate, rat, sheep

Characteristics: Our Interferon-α Receptor, Type I, Subunit I (Ser535,539) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects Interferon-α Receptor, Type I, Subunit I (Ser535,539) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Ser535/539 of human interferon-alpha, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-IFNAR1 antibody (ABIN361505)

Bhattacharya, HuangFu, Liu, Veeranki, Baker, Koumenis, Diehl, Fuchs: "Inducible priming phosphorylation promotes ligand-independent degradation of the IFNAR1 chain of type I interferon receptor." in: The Journal of biological chemistry, Vol. 285, Issue 4, pp. 2318-25, (2010) (PubMed).

Kumar, Krolewski, Fuchs: "Phosphorylation and specific ubiquitin acceptor sites are required for ubiquitination and degradation of the IFNAR1 subunit of type I interferon receptor." in: The Journal of biological chemistry, Vol. 279, Issue 45, pp. 46614-20, (2004) (PubMed).

Target Details for IFNAR1

Target: IFNAR1 (Interferon alpha/beta Receptor 1 (IFNAR1))

Alternative Name: IFNAR1

Background:

Synonyms: Alpha-Type Antiviral Protein, AVP, Beta-Type Antiviral Protein, CRF2-1, Cytokine Receptor Class-II Member 1, Cytokine Receptor Family 2 Member 1, IFN-Alpha/Beta Receptor 1, IFNBR, IFN-Alpha-REC, IFNAR, IFN-R-1, IFRC, Interferon Alpha/Beta Receptor 1, Interferon-Alpha/Beta Receptor Alpha Chain, Interferon Alpha And Beta Receptor Subunit 1, Interferon (Alpha, Beta And Omega) Receptor 1, Interferon-Beta Receptor 1, Interferon Receptor 1, Type I Interferon Receptor 1

Description Logic: Our Interferon-α Receptor, Type I, Subunit I (Ser535,539) rabbit polyclonal phosphospecific primary antibody is produced . It detects Interferon-α Receptor, Type I, Subunit I (Ser535,539) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: IFNAR1

Molecular Weight: 110 kDa-130 kDa

Gene ID: 3654

UniProt: P17181

Pathways: JAK-STAT Signaling, Hepatitis C