MEK1 antibody (pThr386)

Catalog No. ABIN361509

The Rabbit Polyclonal anti-MEK1 antibody (ABIN361509) specifically detects MEK1 in WB and ELISA. The antibody is reactive with Human samples.

Quick Overview for MEK1 antibody (pThr386) (ABIN361509)

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-MEK1 Antibody

Binding Specificity: pThr386

Specificity: Specific for endogenous levels of the ~45 kDa MEK 1 protein phosphorylated at Thr386. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Human, Rat

Predicted Reactivity: bovine, canine, chicken, mouse, non-human primate, Xenopus

Characteristics: Our MEK1 (Thr386) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects MEK1 (Thr386) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr386 of human MEK1, conjugated to keyhole limpet hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

Target Details for MEK1

Target: MEK1 (MAP2K1) (Mitogen-Activated Protein Kinase Kinase 1 (MAP2K1))

Alternative Name: MAP2K1

Background:

Synonyms: Dual specificity mitogen activated protein kinase kinase 1 antibody, Dual specificity mitogen-activated protein kinase kinase 1 antibody, ERK activator kinase 1 antibody, MAP kinase kinase 1 antibody, MAP kinase/Erk kinase 1 antibody, MAP2K1 antibody, MAPK/ERK kinase 1 antibody, MAPKK 1 antibody, MAPKK1 antibody, MEK 1 antibody, Mek1 antibody, MEKK1 antibody, Mitogen activated protein kinase kinase 1 antibody, MKK 1 antibody, MKK1 antibody, MP2K1_HUMAN antibody, PRKMK1 antibody, Protein kinase mitogen activated kinase 1 (MAP kinase kinase 1) antibody, Protein kinase mitogen activated kinase 1 antibody

Description Logic: Our MEK1 (Thr386) rabbit polyclonal phosphospecific primary antibody is produced . It detects MEK1 (Thr386) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: MAP2K1

Molecular Weight: 45 kDa

Gene ID: 5604

UniProt: Q02750

Pathways: MAPK Signaling, RTK Signaling, Interferon-gamma Pathway, Fc-epsilon Receptor Signaling Pathway, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades, Autophagy, Signaling of Hepatocyte Growth Factor Receptor, BCR Signaling