PAK1 antibody (pThr402)

Catalog No. ABIN361511

The Rabbit Polyclonal anti-PAK1 antibody has been validated for WB and ELISA. It is suitable to detect PAK1 in samples from Rat. There is 1 publication available.

Quick Overview for PAK1 antibody (pThr402) (ABIN361511)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-PAK1 Antibody

Binding Specificity: pThr402

Specificity: Specific for endogenous levels of the ~68 kDa PAK protein phosphorylated at Thr402. The immunolabeling is completely eliminated by treatment with λ-phosphatase.

Cross-Reactivity: Mouse, Rat

Predicted Reactivity: bovine, canine, human, mouse

Characteristics: Our PAK-1,2,3 (Thr402) rabbit polyclonal phosphospecific primary antibody is produced in-house. It detects PAK-1,2,3 (Thr402) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Purification: Prepared from pooled rabbit serum by affinity purification via sequential chromatography on phospho and non-phosphopeptide affinity columns.

Immunogen: Synthetic phospho-peptide corresponding to amino acid residues surrounding Thr402 of rat p21 activated kinase 2 (PAK-2), conjugated to keyhole limpet hemocyanin (KLH). The peptide sequence used is identical in PAK-1, 2 and 3. Note: Thr402 in PAK-2 corresponds to Thr423 in human PAK-1.

Isotype: IgG

Application Details

Application Notes:

Dilution Range: WB Brain: 1:1000

Dilution Range: WB: 1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: 10 mM HEPES ( pH 7.5), 150 mM NaCl, 100 μg per ml BSA and 50 % glycerol.

Storage: -20 °C

Storage Comment: Storage at -20°C is recommended, as aliquots may be taken without freeze/thawing due to presence of 50% glycerol. Stable for at least 1 year at -20°C.

Expiry Date: 12 months

References for anti-PAK1 antibody (ABIN361511)

Nie, Sun, Faruque, Ye, Li, Liang, Chang, Yang, Han, Shi: "Synapses of amphids defective (SAD-A) kinase promotes glucose-stimulated insulin secretion through activation of p21-activated kinase (PAK1) in pancreatic β-Cells." in: The Journal of biological chemistry, Vol. 287, Issue 31, pp. 26435-44, (2012) (PubMed).

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1

Background:

Synonyms: Alpha PAK antibody, Beta PAK antibody, bPAK antibody, CDKN1A antibody, Gamma PAK antibody, hPAK3 antibody, MRX30 antibody, MRX47 antibody, Oligophrenin 3 antibody, OPHN3 antibody, P21 (CDKN1A) activated kinase 2 antibody, P21 (CDKN1A) activated kinase 3 antibody, p21 activated kinase 1 antibody, p21 activated kinase 2 antibody, p21 activated kinase 3 antibody, P21 protein (Cdc42/Rac) activated kinase 1 antibody, P21 protein (Cdc42/Rac) activated kinase 2 antibody, P21 protein (Cdc42/Rac) activated kinase 3 antibody, P21/Cdc42/Rac1 activated kinase 1 (STE20 homolog yeast) antibody, P21/Cdc42/Rac1 activated kinase 1 (yeast Ste20 related) antibody, P58 antibody, P65 PAK antibody, PAK 2 antibody, PAK 3 antibody, PAK1 antibody, PAK2 antibody, PAK3 antibody, PAK3beta antibody, PAK65 antibody, PAKalpha antibody, PAKgamma antibody, S6/H4 kinase antibody, Serine/threonine protein kinase PAK 1 antibody, Serine/threonine protein kinase PAK 2 antibody, Serine/threonine protein kinase PAK 3 antibody, Serine/threonine protein kinase PAK1 antibody, Serine/threonine protein kinase PAK2 antibody, Serine/threonine protein kinase PAK3 antibody, STE20 homolog yeast antibody

Description Logic: Our PAK-1,2,3 (Thr402) rabbit polyclonal phosphospecific primary antibody is produced . It detects PAK-1,2,3 (Thr402) and is antigen affinity purified from pooled serum. It is great for use in ELISA, WB.

Gene Name Alternatives: PAK1-3

Molecular Weight: 68 kDa/70 kDa

Gene ID: 29431

UniProt: P35465

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis