PAK1 antibody (AA 401-430)

Catalog No. ABIN3032114

The Rabbit Polyclonal anti-PAK1 antibody is suitable to detect PAK1 in samples from Human. It has been validated for WB, IHC, ELISA and IF.

Quick Overview for PAK1 antibody (AA 401-430) (ABIN3032114)

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This PAK1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Product Details anti-PAK1 Antibody

Binding Specificity: AA 401-430

Purpose: PAK1 Antibody

Cross-Reactivity (Details): Expected species reactivity: Mouse, Rat, Bovine, Rabbit, Xenopus

Purification: Antigen affinity

Immunogen: A portion of amino acids 401-430 from the human protein was used as the immunogen for this PAK1 antibody.

Isotype: Ig Fraction

Application Details

Application Notes: Titration of the PAK1 antibody may be required due to differences in protocols and secondary/substrate sensitivity.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer: In 1X PBS, pH 7.4, with 0.09 % sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Aliquot the PAK1 antibody and store frozen at -20oC or colder. Avoid repeated freeze-thaw cycles.

Target Details for PAK1

Target: PAK1 (P21-Activated Kinase 1 (PAK1))

Alternative Name: PAK1

Background: PAK1, a member of the STE20 subfamily of Ser/Thr protein kinases, acts on a variety of targets. It is likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2. The protein interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. PAK1 binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. It is a component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1. The protein is autophosphorylated when activated by CDC42/p21. Structurally, the PAK1 contains 1 CRIB domain.

UniProt: Q13153

Pathways: MAPK Signaling, RTK Signaling, TCR Signaling, Fc-epsilon Receptor Signaling Pathway, Intracellular Steroid Hormone Receptor Signaling Pathway, Regulation of Intracellular Steroid Hormone Receptor Signaling, Skeletal Muscle Fiber Development, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, Embryonic Body Morphogenesis