SOD1 antibody

Catalog No. ABIN361646

This Rabbit Polyclonal antibody specifically detects SOD1 in WB, IHC, ELISA and IP. It exhibits reactivity toward Human and has been mentioned in 5+ publications.

Quick Overview for SOD1 antibody (ABIN361646)

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SOD1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunoprecipitation (IP)

Product Details anti-SOD1 Antibody

Specificity: Detects ~23 kDa (human) and ~19 kDa (other species).

Cross-Reactivity: Coral, Cow, Dog, Fish, Hamster, Human, Invertebrate, Mollusca, Monkey, Mouse, Pig, Rabbit, Rat, Sheep, Xenopus laevis

Purification: Protein A Purified

Immunogen: Synthetic Human Cu/Zn SOD Peptide

Application Details

Application Notes:

• WB (1:5000)
• IHC (1:100)
• optimal dilutions for assays should be determined by the user.

Comment:

0.2 μg/ml of ABIN361645 was sufficient for detection of Cu/Zn SOD in 20 μg of HeLa cell lysate by colorimetric immunoblot analysis using Goat anti-rabbit IgG:AP as the secondary antibody.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: PBS pH 7.0, 50 % glycerol, 0.09 % sodium azide, Storage buffer may change when conjugated

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: -20°C

References for anti-SOD1 antibody (ABIN361646)

Leung, Wang, Mak, Ng, Leung: "Differential proteomic responses in hepatopancreas and adductor muscles of the green-lipped mussel Perna viridis to stresses induced by cadmium and hydrogen peroxide." in: Aquatic toxicology (Amsterdam, Netherlands), Vol. 105, Issue 1-2, pp. 49-61, (2011) (PubMed).

Wong, Leung, Djurisić, Leung: "Toxicities of nano zinc oxide to five marine organisms: influences of aggregate size and ion solubility." in: Analytical and bioanalytical chemistry, Vol. 396, Issue 2, pp. 609-18, (2010) (PubMed).

Ho, Hsu, Yang, Lee, Chien: "Sialic acid reduces acute endotoxemia-induced liver dysfunction in the rat." in: Shock (Augusta, Ga.), Vol. 32, Issue 2, pp. 228-35, (2009) (PubMed).

Wu, Hsiao, Chien, Lai: "Ischemic conditioning by short periods of reperfusion attenuates renal ischemia/reperfusion induced apoptosis and autophagy in the rat." in: Journal of biomedical science, Vol. 16, pp. 19, (2009) (PubMed).

Lahaie-Collins, Bournival, Plouffe, Carange, Martinoli: "Sesamin modulates tyrosine hydroxylase, superoxide dismutase, catalase, inducible NO synthase and interleukin-6 expression in dopaminergic cells under MPP+-induced oxidative stress." in: Oxidative medicine and cellular longevity, Vol. 1, Issue 1, pp. 54-62, (2009) (PubMed).

Target Details for SOD1

Target: SOD1 (Superoxide Dismutase 1, Soluble (SOD1))

Alternative Name: SOD1

Background: Superoxide dismutase (SOD) is an endogenously produced intracellular enzyme present in almost every cell in the body (3). It works by catalyzing the dismutation of the superoxide radical O2ˉ to O2 and H2O2, which are then metabolized to H2O and O2 by catalase and glutathione peroxidase (2,5). In general, SODs play a major role in antioxidant defense mechanisms (4). There are two main types of SOD in mammalian cells. One form (SOD1) contains Cu and Zn ions as a homodimer and exists in the cytoplasm. The two subunits of 16 kDa each are linked by two cysteines forming an intra-subunit disulphide bridge (3). The second form (SOD2) is a manganese containing enzyme and resides in the mitochondrial matrix. It is a homotetramer of 80 kDa. The third form (SOD3 or EC-SOD) is like SOD1 in that it contains Cu and Zn ions, however it is distinct in that it is a homotetramer, with a mass of 30 kDA and it exists only in the extra-cellular space (7). SOD3 can also be distinguished by its heparin-binding capacity (1).

Gene ID: 6647

NCBI Accession: NP_000445

UniProt: P00441

Pathways: Sensory Perception of Sound, Transition Metal Ion Homeostasis