RPS6KA3 antibody (pThr348)

Catalog No. ABIN362068

The Rabbit Polyclonal anti-RPS6KA3 antibody (ABIN362068) specifically detects RPS6KA3 in WB, IHC and IF. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for RPS6KA3 antibody (pThr348) (ABIN362068)

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RPS6KA3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-RPS6KA3 Antibody

Binding Specificity: pThr348

Specificity: The antibody detects endogenous level of p90RSK only when phosphorylated at threonine 348.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pThr348 (S-R-T (p) -P-R) derived from Human p90RSK. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for RPS6KA3

Target: RPS6KA3 (Ribosomal Protein S6 Kinase, 90kDa, Polypeptide 3 (RPS6KA3))

Alternative Name: p90RSK

Background: Serine/threonine kinase that may play a role in mediating the growth-factor and stress induced activation of the transcription factor CREB.

Molecular Weight: 90 kDa

NCBI Accession: NP_001006

UniProt: Q15418

Pathways: MAPK Signaling, Neurotrophin Signaling Pathway, Activation of Innate immune Response, Toll-Like Receptors Cascades