beta Catenin antibody (pSer45)
Quick Overview for beta Catenin antibody (pSer45) (ABIN362090)
Target
See all beta Catenin (CATNB) AntibodiesReactivity
Host
Clonality
Conjugate
Application
-
-
Binding Specificity
- pSer45
-
Specificity
- The antibody detects endogenous level of b-Catenin only when phosphorylated at threonine 41/serine 45.
-
Purification
- The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
-
Immunogen
- Peptide sequence around phosphorylation site of pThr 41, pSer45 (A-T-T (p) -T-A-P-S (p) -L-S) derived from Human b-Catenin. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
-
Isotype
- IgG
-
-
-
-
Application Notes
- Western blotting: 1:500-1:1000
-
Restrictions
- For Research Use only
-
-
-
Format
- Liquid
-
Concentration
- 1 mg/mL
-
Buffer
- Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
-
Preservative
- Sodium azide
-
Precaution of Use
- This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
-
Storage
- 4 °C/-20 °C
-
Storage Comment
- Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
-
-
- beta Catenin (CATNB) (Catenin, beta (CATNB))
-
Alternative Name
- beta-Catenin
-
Background
- Involved in the regulation of cell adhesion and in signal transduction through the Wnt pathway.
-
Molecular Weight
- 92 kDa
-
NCBI Accession
- NP_001091
-
UniProt
- P35222
-
Pathways
- Peptide Hormone Metabolism
Target
-