Cofilin antibody (pSer3)

Catalog No. ABIN362130

There are 4+ publications for this product available. The Rabbit Polyclonal anti-Cofilin antibody is suitable to detect Cofilin in samples from Human, Mouse and Rat. It has been validated for WB, IHC and IF.

Quick Overview for Cofilin antibody (pSer3) (ABIN362130)

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Cofilin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunofluorescence (IF)

Product Details anti-Cofilin Antibody

Binding Specificity: pSer3

Specificity: The antibody detects endogenous level of cofilin only when phosphorylated at serine 3.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer3 (M-A-S (p) -G-V) derived from Human cofilin. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Immunofluorescence: 1:100-1:200

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

References for anti-Cofilin antibody (ABIN362130)

Huet, Rajakylä, Viita, Skarp, Crivaro, Dopie, Vartiainen: "Actin-regulated feedback loop based on Phactr4, PP1 and cofilin maintains the actin monomer pool." in: Journal of cell science, Vol. 126, Issue Pt 2, pp. 497-507, (2013) (PubMed).

Zhang, Luo, Wan, Wu, Laski, Chen: "Regulation of cofilin phosphorylation and asymmetry in collective cell migration during morphogenesis." in: Development (Cambridge, England), Vol. 138, Issue 3, pp. 455-64, (2011) (PubMed).

Guo, Lv, Tian, Hu, Wang, Sui, Jiang, Ruan, Nan: "Downregulation of p57 accelerates the growth and invasion of hepatocellular carcinoma." in: Carcinogenesis, Vol. 32, Issue 12, pp. 1897-904, (2011) (PubMed).

Scott: "H. R. Morgan, Astronomer." in: Science (New York, N.Y.), Vol. 126, Issue 3272, pp. 497, (2010) (PubMed).

Target Details for Cofilin

Target: Cofilin (CFL1) (Cofilin 1 (CFL1))

Alternative Name: cofilin

Background: Controls reversibly actin polymerization and depolymerization in a pH-sensitive manner. It has the ability to bind G- and F-actin in a 1:1 ratio of cofilin to actin. It is the major component of intranuclear and cytoplasmic actin rods.

Molecular Weight: 19 kDa

NCBI Accession: NP_005498

UniProt: P23528

Pathways: Regulation of Cell Size, Tube Formation, Protein targeting to Nucleus, CXCR4-mediated Signaling Events