MAP3K5 antibody (Mitogen-Activated Protein Kinase Kinase Kinase 5) (pSer966)

Details for Product anti-MAP3K5 Antibody No. ABIN362196, Supplier: Log in to see
Antigen
  • ASK1
  • MAPKKK5
  • MEKK5
  • mitogen-activated protein kinase kinase kinase 5
  • MUD21.11
  • MUD21_11
  • 7420452D20Rik
  • ASK
  • Mekk5
  • Ask1
  • RGD1306565
  • mitogen-activated protein kinase kinase kinase 5
  • MAP3K5
  • MAPKKK5
  • Map3k5
  • map3k5
Alternatives
anti-Human MAP3K5 antibody for Western Blotting
Epitope
pSer966
64
51
30
23
19
18
15
15
15
10
10
9
7
6
6
5
5
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4
3
3
3
3
3
3
2
2
2
2
2
2
1
1
1
1
1
1
1
1
1
1
1
Reactivity
Human, Mouse (Murine)
400
176
97
5
1
1
Host
Rabbit
378
28
Clonality
Polyclonal
Conjugate
This MAP3K5 antibody is un-conjugated
11
11
10
5
5
5
5
5
5
5
5
5
5
5
4
4
4
2
Application
Immunohistochemistry (IHC), Western Blotting (WB)
312
172
135
83
65
45
21
14
12
11
8
4
2
1
1
Options
Supplier
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Immunogen Peptide sequence around phosphorylation site of pSer966 (S-I-S (p) -L-P) derived from Human ASK1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
Isotype IgG
Specificity The antibody detects endogenous level of ASK1 only when phosphorylated at serine 966.
Purification The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatography using non-phosphopeptide corresponding to the phosphorylation site.
Plasmids, Primers & others Plasmids, Primers & others MAP3K5 products on genomics-online (e.g. as negative or positive controls)
Alternative Name ASK1 (MAP3K5 Antibody Abstract)
Background Component of a protein kinase signal transduction cascade. Phosphorylates and activates MAP2K4 and MAP2K6, which in turn activate the JNK and p38 MAP kinases, respectively.
Molecular Weight 155 kDa
NCBI Accession NP_005914
UniProt Q99683
Pathways MAPK Signaling, Positive Regulation of Endopeptidase Activity, Unfolded protein response
Application Notes Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100
Restrictions For Research Use only
Format Liquid
Concentration 1 mg/mL
Buffer Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
Preservative Sodium azide
Precaution of Use This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage 4 °C/-20 °C
Storage Comment Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Product cited in: Yang, Yu, Guan, Gu, Cao, Wang, Zheng, Xu, Shen, Liu: "ASK1-JNK signaling cascade mediates Ad-ST13-induced apoptosis in colorectal HCT116 cells." in: Journal of cellular biochemistry, Vol. 110, Issue 3, pp. 581-8, 2010 (PubMed).

Background publications Zhang, Zheng, Storz, Min: "Protein kinase D specifically mediates apoptosis signal-regulating kinase 1-JNK signaling induced by H2O2 but not tumor necrosis factor." in: The Journal of biological chemistry, Vol. 280, Issue 19, pp. 19036-44, 2005 (PubMed).

Fujii, Goldman, Park, Zhang, Chen, Fu: "Negative control of apoptosis signal-regulating kinase 1 through phosphorylation of Ser-1034." in: Oncogene, Vol. 23, Issue 29, pp. 5099-104, 2004 (PubMed).

Goldman, Chen, Fu: "Activation of apoptosis signal-regulating kinase 1 by reactive oxygen species through dephosphorylation at serine 967 and 14-3-3 dissociation." in: The Journal of biological chemistry, Vol. 279, Issue 11, pp. 10442-9, 2004 (PubMed).

Zhang, Chen, Fu: "Suppression of apoptosis signal-regulating kinase 1-induced cell death by 14-3-3 proteins." in: Proceedings of the National Academy of Sciences of the United States of America, Vol. 96, Issue 15, pp. 8511-5, 1999 (PubMed).

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