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Paxillin antibody (pTyr31)

PXN Reactivity: Human WB Host: Rabbit Polyclonal unconjugated
Catalog No. ABIN362228
  • Target See all Paxillin (PXN) Antibodies
    Paxillin (PXN)
    Binding Specificity
    • 32
    • 26
    • 17
    • 16
    • 11
    • 9
    • 8
    • 7
    • 7
    • 7
    • 5
    • 4
    • 3
    • 3
    • 3
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 2
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    • 1
    pTyr31
    Reactivity
    • 199
    • 104
    • 72
    • 16
    • 8
    • 7
    • 5
    • 4
    • 3
    • 3
    • 1
    • 1
    • 1
    • 1
    • 1
    Human
    Host
    • 191
    • 27
    • 1
    Rabbit
    Clonality
    • 176
    • 43
    Polyclonal
    Conjugate
    • 122
    • 10
    • 8
    • 8
    • 8
    • 8
    • 8
    • 8
    • 6
    • 6
    • 4
    • 4
    • 4
    • 4
    • 4
    • 2
    • 2
    • 2
    • 1
    This Paxillin antibody is un-conjugated
    Application
    • 167
    • 67
    • 41
    • 40
    • 37
    • 28
    • 27
    • 25
    • 18
    • 14
    • 14
    • 6
    • 2
    • 1
    • 1
    • 1
    Western Blotting (WB)
    Specificity
    The antibody detects endogenous level of Paxillin only when phosphorylated at tyrosine 31.
    Purification
    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.
    Immunogen
    Peptide sequence around phosphorylation site of pTyr31 (T-P-Y (p) -S-Y) derived from Human Paxillin. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.
    Isotype
    IgG
    Top Product
    Discover our top product PXN Primary Antibody
  • Application Notes
    Western blotting: 1:500-1:1000
    Restrictions
    For Research Use only
  • Format
    Liquid
    Concentration
    1 mg/mL
    Buffer
    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.
    Preservative
    Sodium azide
    Precaution of Use
    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C/-20 °C
    Storage Comment
    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Huang, Yan, Ge: "JNK regulates cell migration through promotion of tyrosine phosphorylation of paxillin." in: Cellular signalling, Vol. 20, Issue 11, pp. 2002-12, (2008) (PubMed).

  • Target
    Paxillin (PXN)
    Alternative Name
    Paxillin (PXN Products)
    Synonyms
    AW108311 antibody, AW123232 antibody, Pax antibody, CG18061 antibody, CG18576 antibody, CG31794 antibody, CT40481 antibody, CT42454 antibody, DPaxillin antibody, DPxn antibody, DPxn37 antibody, Dmel\\CG31794 antibody, Dpax antibody, DpaxA antibody, PDLP antibody, dPax antibody, pax antibody, wu:fw71f12 antibody, PXN antibody, LOC100220858 antibody, paxillin antibody, Paxillin antibody, paxillin S homeolog antibody, paxillin a antibody, PXN antibody, Pxn antibody, Pax antibody, pxn.S antibody, pxna antibody, LOC696336 antibody, pxn antibody
    Background
    An antiphosphotyrosine antibody was used to identify proteins that are phosphorylated in Rous sarcoma virus-transformed chick embryo fibroblasts, and a 76-kD protein was obtained that localizes to focal adhesions at the ends of actin-containing stress fibers in nontransformed cells (Ref.1). This protein was purified from chicken gizzard smooth muscle, and was named Pxn (Paxillin) ('paxillus' means 'small stake' or 'peg' in Latin) as a protein tethered to the membrane at focal adhesions
    Molecular Weight
    68 kDa
    NCBI Accession
    NP_001074
    UniProt
    P49023
    Pathways
    MAPK Signaling, EGFR Signaling Pathway, Response to Growth Hormone Stimulus, Cell-Cell Junction Organization, Maintenance of Protein Location, CXCR4-mediated Signaling Events, Signaling Events mediated by VEGFR1 and VEGFR2, Signaling of Hepatocyte Growth Factor Receptor, VEGF Signaling
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