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Ezrin antibody (pThr567)

Cited in 1 publication. The Rabbit Polyclonal anti-Ezrin antibody (ABIN362230) specifically detects Ezrin in WB. The antibody is reactive with Human, Mouse and Rat samples.

(1 reference)

Catalog No. ABIN362230

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Quick Overview for Ezrin antibody (pThr567) (ABIN362230)

Target

See all Ezrin (EZR) Antibodies

Ezrin (EZR)

Reactivity

Human, Mouse, Rat

Host

Rabbit

Clonality

Polyclonal

Conjugate

This Ezrin antibody is un-conjugated

Application

Western Blotting (WB)

Discover our top product EZR Primary Antibody

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pThr567

Specificity

The antibody detects endogenous level of Ezrin only when phosphorylated at threonine 567.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen

Peptide sequence around phosphorylation site of pThr567 (Y-K-T (p) -L-R) derived from Human Ezrin. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype

IgG
anti-Ezrin (EZR) (AA 292-464) antibody
EZR Reactivity: Human, Mouse, Monkey WB, ELISA, ICC, FACS Host: Mouse Monoclonal 6F1A9 unconjugated

anti-Ezrin (EZR) (AA 1-295) antibody
EZR Reactivity: Human WB, IHC, IP, ICC Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (AA 1-586) antibody
EZR Reactivity: Human WB, IP Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (pTyr478) antibody
EZR Reactivity: Human, Mouse, Rat WB, ELISA, IHC, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (AA 1-586) antibody
EZR Reactivity: Human WB, ELISA, IHC (p), PLA Host: Mouse Monoclonal 1E11 unconjugated

anti-Ezrin (EZR) (pTyr353) antibody
EZR Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) antibody
EZR Reactivity: Human WB Host: Mouse Monoclonal CPTC-Ezrin-1 unconjugated

anti-Ezrin (EZR) (N-Term) antibody
EZR Reactivity: Human, Mouse, Rat, Monkey WB, IHC, IF, ICC, FACS Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (pThr567) antibody
EZR Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

anti-Ezrin (EZR) (C-Term) antibody
EZR Reactivity: Human, Mouse, Rat WB, ELISA, IF, ICC Host: Rabbit Polyclonal unconjugated

Application Notes

Western blotting: 1:500-1:1000

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Cui, Li, Zhang, Han: "Expression of Ezrin and phosphorylated Ezrin (pEzrin) in pancreatic ductal adenocarcinoma." in: Cancer investigation, Vol. 28, Issue 3, pp. 242-7, (2010) (PubMed).
Target

Ezrin (EZR)

Alternative Name

Ezrin

Background

Probably involved in connections of major cytoskeletal structures to the plasma membrane. In epithelial cells, required for the formation of microvilli and membrane ruffles on the apical pole. Along with PLEKHG6, required for normal macropinocytosis.

Molecular Weight

81 kDa

NCBI Accession

NP_001104

UniProt

P15311

Pathways

Maintenance of Protein Location