RAC1 antibody (pSer71)

Catalog No. ABIN362232

The Rabbit Polyclonal anti-RAC1 antibody is suitable to detect RAC1 in samples from Human, Mouse and Rat. It has been validated for WB.

Quick Overview for RAC1 antibody (pSer71) (ABIN362232)

Target: RAC1

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This RAC1 antibody is un-conjugated

Application: Western Blotting (WB)

Product Details anti-RAC1 Antibody

Binding Specificity: pSer71

Specificity: The antibody detects endogenous level of Rac1 only when phosphorylated at serine 71.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer71 (P-L-S (p) -Y-P) derived from Human Rac1. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for RAC1

Target: RAC1

Background: Rac1 encoded by this gene is a GTPase which belongs to the RAS superfamily of small GTP-binding proteins. Members of this superfamily appear to regulate a diverse array of cellular events, including the control of cell growth, cytoskeletal reorganization, and the activation of protein kinases. Two transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 28 kDa

NCBI Accession: NP_008839

UniProt: P63000

Pathways: WNT Signaling, Regulation of Actin Filament Polymerization, Cell-Cell Junction Organization, Thromboxane A2 Receptor Signaling, VEGF Signaling