MEK1 + MEK2 antibody (pSer217, pSer221)

Catalog No. ABIN362236

The Rabbit Polyclonal anti-MEK1 + MEK2 antibody (ABIN362236) specifically detects MEK1 + MEK2 in IHC. The antibody is reactive with Human, Mouse and Rat samples.

Quick Overview for MEK1 + MEK2 antibody (pSer217, pSer221) (ABIN362236)

Target: MEK1 + MEK2

Reactivity: Human, Mouse, Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MEK1 + MEK2 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Product Details anti-MEK1 + MEK2 Antibody

Binding Specificity: pSer217, pSer221

Specificity: The antibody detects endogenous level of MEK1/MEK2 only when phosphorylated at serine 217/221.

Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography usingepitope-specific phosphopeptide. The antibody against non-phosphopeptide was removedby chromatogramphy using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen: Peptide sequence around phosphorylation site of pSer217, 221 (I-D-S (p) -M-A) derived from Human MEK1, MEK2. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype: IgG

Application Details

Application Notes: Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative: Sodium azide

Precaution of Use: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C/-20 °C

Storage Comment: Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.

Target Details for MEK1 + MEK2

Target: MEK1 + MEK2

Alternative Name: MEK1/MEK2

Background: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates ERK1 and ERK2 MAP kinases.

Molecular Weight: 45 kDa

UniProt: Q02750