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HSP27 antibody (pSer82)

This Rabbit Polyclonal antibody specifically detects HSP27 in WB and IHC. It exhibits reactivity toward Human.
Catalog No. ABIN362306
$350.90
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Quick Overview for HSP27 antibody (pSer82) (ABIN362306)

Target

See all HSP27 (HSPB1) Antibodies
HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity

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Human

Host

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Rabbit

Clonality

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Polyclonal

Conjugate

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This HSP27 antibody is un-conjugated

Application

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Western Blotting (WB), Immunohistochemistry (IHC)
  • Binding Specificity

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    pSer82

    Specificity

    The antibody detects endogenous level of HSP27 only when phosphorylated at serine 82.

    Purification

    The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

    Immunogen

    Peptide sequence around phosphorylation site of pSer82 (Q-L-S (p) -S-G) derived from Human HSP27. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

    Isotype

    IgG
  • Application Notes

    Western blotting: 1:500-1:1000
    Immunohistochemistry: 1:50-1:100

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    1 mg/mL

    Buffer

    Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C/-20 °C

    Storage Comment

    Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
  • Target

    HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

    Alternative Name

    HSP27

    Background

    Involved in stress resistance and actin organization.

    Molecular Weight

    27 kDa

    NCBI Accession

    NP_001531

    UniProt

    P04792

    Pathways

    MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling
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