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HSP27 antibody (pSer82)

This Rabbit Polyclonal antibody specifically detects HSP27 in WB and IHC. It exhibits reactivity toward Human.

Catalog No. ABIN362306

Datasheet as PDF

Quick Overview for HSP27 antibody (pSer82) (ABIN362306)

Target

See all HSP27 (HSPB1) Antibodies

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Reactivity

Human

Host

Rabbit

Clonality

Polyclonal

Conjugate

This HSP27 antibody is un-conjugated

Application

Western Blotting (WB), Immunohistochemistry (IHC)

Discover our top product HSPB1 Primary Antibody

Binding Specificity
42

34

31

16

16

13

8

8

8

6

6

6

4

4

3

2

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
pSer82

Specificity

The antibody detects endogenous level of HSP27 only when phosphorylated at serine 82.

Purification

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Immunogen

Peptide sequence around phosphorylation site of pSer82 (Q-L-S (p) -S-G) derived from Human HSP27. Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates.

Isotype

IgG
anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 101-205) antibody
HSPB1 Reactivity: Human, Mouse, Rat WB, ELISA, FACS, IHC (p), IF (cc), IF (p), IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human, Rat WB, IHC, ELISA, FACS, ICC Host: Mouse Monoclonal 5D7 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human WB, IHC, ELISA, IF, FACS, IP Host: Rabbit Monoclonal 10C11 unconjugated Recombinant Antibody

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human, Mouse, Rat, Sheep, Chimpanzee, Chicken, Monkey WB, IHC, IF, FACS, ISt, StM Host: Mouse Monoclonal G3-1 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human WB, IHC, IF, FACS, IP, ICC Host: Mouse Monoclonal 8A7 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) (Center) antibody
HSPB1 Reactivity: Human, Mouse, Rat WB, IF, FACS, IP, IHC (p), ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) (AA 96-205) antibody
HSPB1 Reactivity: Human WB, ELISA, IF, IP, PLA Host: Mouse Monoclonal 3G3 unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) (pSer82) antibody
HSPB1 Reactivity: Human WB, IHC, IP Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human WB, IHC, IF, FACS, ICC Host: Rabbit Polyclonal unconjugated

anti-Heat Shock 27kDa Protein 1 (HSPB1) antibody
HSPB1 Reactivity: Human WB, IHC, ELISA, IF, IP, ICC Host: Mouse Monoclonal 5D12-A12 unconjugated

Application Notes

Western blotting: 1:500-1:1000
Immunohistochemistry: 1:50-1:100

Restrictions

For Research Use only
Format

Liquid

Concentration

1 mg/mL

Buffer

Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150 mM NaCl, 0.02 % sodium azide and 50 % glycerol.

Preservative

Sodium azide

Precaution of Use

This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage

4 °C/-20 °C

Storage Comment

Store at -20 °C for long term preservation (recommended). Store at 4 °C for short term use.
Target

HSP27 (HSPB1) (Heat Shock 27kDa Protein 1 (HSPB1))

Alternative Name

HSP27

Background

Involved in stress resistance and actin organization.

Molecular Weight

27 kDa

NCBI Accession

NP_001531

UniProt

P04792

Pathways

MAPK Signaling, Regulation of Actin Filament Polymerization, Signaling Events mediated by VEGFR1 and VEGFR2, Negative Regulation of intrinsic apoptotic Signaling, VEGF Signaling